Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus
| Autor: | Frank Laue, Christoph Kessler, Karl O. Stetter, Kurt Schmid, Rüdiger Schmitt, Axel Laminet, Michael Thomm |
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| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
Genetics
chemistry.chemical_classification Base Sequence viruses DNA Restriction Enzymes Euryarchaeota Biology Cleavage (embryo) PBR322 Virus-SV40 Substrate Specificity Kinetics chemistry.chemical_compound Restriction enzyme Enzyme Plasmid chemistry Methanococcus aeolicus 570 Biowissenschaften Biologie ddc:570 Deoxyribonucleases Type II Site-Specific DNA |
| Popis: | Three type II restriction endonucleases, MaeI, MaeII and MaeIII, with novel site specificities have been isolated and purified from the archaebacterium Methanococcus aeolicus PL-15/H. The recognition sequences of these enzymes are (formula: see text) with the sites of cleavage as indicated by the arrows. The sequences were confirmed by restriction and computer analyses on sequenced DNA's of plasmid pBR322, bacteriophages lambda and phi X174 and virus SV40. |
| Databáze: | OpenAIRE |
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