Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance
| Autor: | Alexander van der Bourg, Maya Mills, Amy S. Chuong, Adrian Cheng, Andrea Benucci, Atsushi Miyawaki, Ladan Egolf, Matteo Carandini, Nathan C. Klapoetke, Fritjof Helmchen, Bosiljka Tasic, Edward S. Boyden, Susan M. Sunkin, Lu Li, R. Clay Reid, Yusuke Niino, Andras Nagy, Claudio Monetti, Ruth M. Empson, Hong Gu, Linda Madisen, Thomas Knöpfel, Thuc Nghi Nguyen, Aleena R. Garner, Hongkui Zeng, Daisuke Shimaoka |
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| Přispěvatelé: | University of Zurich, Zeng, Hongkui, Massachusetts Institute of Technology. Media Laboratory, McGovern Institute for Brain Research at MIT, Chuong, Amy S, Klapoetke, Nathan Cao, Boyden, Edward |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genetically modified mouse
0303 health sciences 10242 Brain Research Institute Effector General Neuroscience Transgene Neuroscience(all) 2800 General Neuroscience Locus (genetics) 610 Medicine & health Computational biology Optogenetics Biology 3. Good health Viral vector 03 medical and health sciences 0302 clinical medicine Gene expression Recombinase 570 Life sciences biology Neuroscience 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | PMC ResearcherID |
| DOI: | 10.5167/uzh-117185 |
| Popis: | available in PMC 2016 March 04 An increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA-dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at substantially higher levels than before. High functionality was shown in example mouse lines for GCaMP6, YCX2.60, VSFP Butterfly 1.2, and Jaws. These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity. National Institutes of Health (U.S.) (NIH grant DA028298) Wellcome Trust (London, England) (Grant) National Institutes of Health (U.S.) (NIH grant MH085500) |
| Databáze: | OpenAIRE |
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