Pandanus tectorius fruit extract promotes Hsp70 accumulation, immune-related genes expression and Vibrio parahaemolyticus tolerance in the white-leg shrimp Penaeus vannamei
| Autor: | Mat Taib Mimi Iryani, Muhd Danish-Daniel, Anupa Anirudhan, Yeong Yik Sung, Patrick Sorgeloos, Tengku Sifzizul Tengku-Muhammad, Li Lian Wong, Yosie Andriani, Victor Tosin Okomoda, Mohd Effendy Abd Wahid, Min Pau Tan, Wen Jye Mok |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pandanus tectorius Aquatic Science Median lethal dose Microbiology 03 medical and health sciences Penaeidae Environmental Chemistry Animals HSP70 Heat-Shock Proteins Penaeus Pandanaceae biology Macrobrachium rosenbergii Plant Extracts Vibrio parahaemolyticus 04 agricultural and veterinary sciences General Medicine biology.organism_classification Animal Feed Vibrio Immunity Innate Shrimp Diet 030104 developmental biology Fruit 040102 fisheries 0401 agriculture forestry and fisheries Hepatopancreas |
| Zdroj: | Fishshellfish immunology. 109 |
| ISSN: | 1095-9947 |
| Popis: | Plants and herbal extracts are indispensable for controlling the spread of disease-causing bacteria, including those that infect aquatic organisms used in aquaculture. The use of plant or herbal extract is expected to be safe for aquatic animals and less harmful to the environment, as opposed to conventional therapeutic alternatives such as antibiotics that promote the occurrence of potential antibiotic-resistant bacteria when used improperly. The efficacy of Pandanus tectorius fruit extract in the regulation of Hsp70 expression, pro-phenoloxidase (ProPO), peroxinectin, penaeidin, crustin and transglutaminase, all immune peptides essential for Vibrio tolerance in white leg shrimp, Penaeus vannamei, was investigated in this study, which included the determination of the safety levels of the extract. Tolerance of shrimp against Vibrio parahaemolyticus, a pathogenic bacteria that causes Acute Hepatopancreas Necrosis Disease (AHPND), was assessed on the basis of median lethal dose challenge survival (LD50 = 106 cells/ml). Mortality was not observed 24 h after exposure of 0.5–6 g/L of the fruit extract, indicating that P. tectorius was not toxic to shrimp at these concentrations. A 24-h incubation of 2–6 g/L of the fruit extract increased shrimp tolerance to V. parahaemolyticus, with survival doubled when the maximum dose tested in this study was used. Concomitant with a rise in survival was the increase in immune-related proteins, with Hsp70, ProPO, peroxinectin, penaeidin, crustin and transglutaminase increased 10, 11, 11, 0.4, 8 and 13-fold respectively. Histological examination of the hepatopancreas and muscle tissues of Vibrio-infected shrimp primed with P. tectorius extract revealed reduced signs of histopathological degeneration, possibly due to the accumulation of Hsp70, a molecular chaperone crucial to cellular protein folding, tissue repair and immune response of living organisms, including Penaeid shrimp. |
| Databáze: | OpenAIRE |
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