Development of a novel method to determine very low density lipoprotein kinetics
| Autor: | Iqbal A. R. Al-Shayji, Muriel J. Caslake, Jason M.R. Gill, Samira Siddiqui, Josephine Cooney |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male medicine.medical_specialty Very low-density lipoprotein Fat Emulsions Intravenous Apolipoprotein B clearance QD415-436 Lipoproteins VLDL Biochemistry chemistry.chemical_compound Endocrinology Insulin resistance Internal medicine Chylomicrons medicine Humans apolipoprotein B triglyceride Triglycerides Apolipoproteins B Lipoprotein lipase biology Triglyceride Chemistry Cell Biology Middle Aged medicine.disease Kinetics Intralipid biology.protein lipids (amino acids peptides and proteins) Female production Clearance rate Chylomicron Lipoprotein |
| Zdroj: | Journal of Lipid Research, Vol 48, Iss 9, Pp 2086-2095 (2007) |
| ISSN: | 0022-2275 |
| Popis: | Isotopic tracer methods of determining triglyceride-rich lipoprotein (TRL) kinetics are costly, time-consuming, and labor-intensive. This study aimed to develop a simpler and cost-effective method of obtaining TRL kinetic data, based on the fact that chylomicrons compete with large VLDL (VLDL(1); S(f) = 60-400) for the same catalytic pathway. Ten healthy subjects [seven men; fasting triglyceride (TG), 44.3-407.6 mg/dl; body mass index, 21-35 kg/m(2)] were given an intravenous infusion of a chylomicron-like TG emulsion (Intralipid; 0.1 g/kg bolus followed by 0.1 g/kg/h infusion) for 75-120 min to prevent the clearance of VLDL(1) by lipoprotein lipase. Multiple blood samples were taken during and after infusion for separation of Intralipid, VLDL(1), and VLDL(2) by ultracentrifugation. VLDL(1)-apolipoprotein B (apoB) and TG production rates were calculated from their linear increases in the VLDL(1) fraction during the infusion. Intralipid-TG clearance rate was determined from its exponential decay after infusion. The production rates of VLDL(1)-apoB and VLDL(1)-TG were (mean +/- SEM) 25.4 +/- 3.9 and 1,076.7 +/- 224.7 mg/h, respectively, and the Intralipid-TG clearance rate was 66.9 +/- 11.7 pools/day. Kinetic data obtained from this method agree with values obtained from stable isotope methods and show the expected relationships with indices of body fatness and insulin resistance (all P < 0.05). The protocol is relatively quick, inexpensive, and transferable to nonspecialist laboratories. |
| Databáze: | OpenAIRE |
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