EFFECT OF BLEEDING TO NERVE REGENERATION AND EPINEURAL SCAR FORMATION IN RAT SCIATIC NERVES (EXPERIMENTAL STUDY)
| Autor: | Halil Bekler, Turhan Özler, Erkan Servet, Afşar Timuçin Özkut, Volkan Kilincoglu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Hemorrhage Tissue Adhesions Electromyography Sodium Chloride Rats Sprague-Dawley Cicatrix Peripheral Nerve Injuries Animals Medicine Orthopedics and Sports Medicine Saline Wound Healing Tissue Adhesion medicine.diagnostic_test Heparin Plant Extracts business.industry Regeneration (biology) General Medicine Sciatic Nerve Nerve Regeneration Rats Surgery Disease Models Animal Diffusion Tensor Imaging Anesthesia Peripheral nerve injury Sciatic nerve business Wound healing medicine.drug |
| Zdroj: | ACTA ORTHOPAEDICA et TRAUMATOLOGICA TURCICA. |
| ISSN: | 1017-995X |
| Popis: | Objective: Epineural scar formation is one of the most significant negative factors affecting surgical repair after peripheral nerve injury. The scar tissue mechanically hinders axonal regeneration and causes adhesions between nerves and surrounding tissues. A hemostatic agent Ankaferd Blood Stopper (ABS; Immun Gida Ilac Kozmetik San. ve Tic. Ltd. Sti., Istanbul, Turkey) has not been previously used. Decreasing the postoperative bleeding and adhesions between nerve and surrounding tissues will prevent the formation of scar tissue, as well as corresponding compressive neuropathy and/or deceleration of axonal regeneration. The purpose of this experimental study was to investigate the effects of bleeding on nerve healing and scar tissue after repair of peripheral nerve injuries. Methods: The right sciatic nerve of 30 Sprague-Dawley male rats (weighing 260–330 g) was cut 1.5 cm proximal to the trifurcation and repaired primarily with 8/0 sutures using epineural technique. The rats were then divided into 3 groups. Saline was applied in Group 1 (n=10), ABS in Group 2 (n=10), and heparin in Group 3 (n=10) for 5 minutes to the repair site and surrounding tissues. In each group, electrophysiological measurements were performed with electromyography (EMG) at postoperative week 12. Magnetic resonance diffusion tensor imaging was used at week 12. Macroscopical and histopathological evaluations were conducted after sacrificing the rats at week 24 with total excision of the repaired sciatic nerves and surrounding tissues. Results: The ABS and saline groups showed better healing than the heparin group. The ABS and saline groups were better in the histopathologic evaluations, but there was no statistically significant difference between the 2 groups. Conclusion: Statistically significant differences were not found between the 3 groups. Significant results may be obtained with larger studies. |
| Databáze: | OpenAIRE |
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