Immunogenic Properties of alpha(1 6) Dextran, Its Protein Conjugates, and Conjugates of Its Breakdown Products in Mice
| Autor: | I G Outschoorn, Ilkka Seppälä, Olli Mäkelä, A W Richter, F Péterfy |
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| Rok vydání: | 1984 |
| Předmět: |
Male
Antigenicity Freund's Adjuvant Immunology Serum albumin Antibodies Mice chemistry.chemical_compound Antigen Animals Antigens Bovine serum albumin Mice Inbred BALB C biology Immunogenicity Albumin Dextrans Serum Albumin Bovine Radioimmunoassay General Medicine Molecular biology Molecular Weight Dextran chemistry Biochemistry Antibody Formation Mice Inbred CBA biology.protein Female Carrier Proteins Haptens |
| Zdroj: | Scandinavian Journal of Immunology. 19:541-550 |
| ISSN: | 1365-3083 0300-9475 |
| DOI: | 10.1111/j.1365-3083.1984.tb00965.x |
| Popis: | Mice were immunized with alpha (1-6) dextran, either as such or coupled to protein carriers, and their anti-dextran response was measured by a solid-phase radioimmunoassay and the Farr assay. Like earlier investigators we found that protein-conjugated dextran was more antigenic than plain dextran. Our novel findings were that (1) a standard dose (30 micrograms of dextran per injection) coupled to strongly antigenic protein (chicken serum albumin (CSA) was three times more antigenic than dextran coupled to weakly antigenic bovine serum albumin (BSA); (2) dextrans of low molecular weight (1000-10,000 daltons) coupled to CSA induced at least ten times stronger secondary responses than did a similarly coupled macromolecular dextran (5-40 million daltons); (3) variation of the CHO/protein ratio from 0.3 to 1 had little effect on the antigenicity of the dextran. Increase of the ratio from one appeared to decrease immunogenicity when BSA was the carrier but not when CSA was the carrier. |
| Databáze: | OpenAIRE |
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