Characterization of a New Chronic Lymphocytic Leukemia Cell Line for Mechanistic In Vitro and In Vivo Studies Relevant to Disease
| Autor: | Nyla A. Heerema, Amy S. Ruppert, Laura Z. Rassenti, Leigha Senter, Amy J. Johnson, David E. Symer, Leslie A. Andritsos, Timothy L. Chen, William H. Towns, Amy Lehman, Arletta Lozanski, Tiina M. Järvinen, Albert de la Chapelle, Weiqiang Zhao, Brad Bolon, John C. Byrd, Kyle A. Beckwith, Gerard Lozanski, Carlo M. Croce, Erin Hertlein |
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| Rok vydání: | 2013 |
| Předmět: |
Herpesvirus 4
Human Chronic lymphocytic leukemia Cell Culture Techniques lcsh:Medicine Biology Flow cytometry Mice 03 medical and health sciences 0302 clinical medicine Immunophenotyping Cell Movement immune system diseases Cell Line Tumor hemic and lymphatic diseases medicine Animals Humans lcsh:Science 030304 developmental biology 0303 health sciences Multidisciplinary medicine.diagnostic_test Microarray analysis techniques lcsh:R Cell Transformation Viral medicine.disease Leukemia Lymphocytic Chronic B-Cell 3. Good health Leukemia Phenotype medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Immunology Cancer research lcsh:Q Bone marrow Immortalised cell line Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 10, p e76607 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Studies of chronic lymphocytic leukemia (CLL) have yielded substantial progress, however a lack of immortalized cell lines representative of the primary disease has hampered a full understanding of disease pathogenesis and development of new treatments. Here we describe a novel CLL cell line (OSU-CLL) generated by EBV transformation, which displays a similar cytogenetic and immunophenotype observed in the patient's CLL (CD5 positive with trisomy 12 and 19). A companion cell line was also generated from the same patient (OSU-NB). This cell line lacked typical CLL characteristics, and is likely derived from the patient's normal B cells. In vitro migration assays demonstrated that OSU-CLL exhibits migratory properties similar to primary CLL cells whereas OSU-NB has significantly reduced ability to migrate spontaneously or towards chemokine. Microarray analysis demonstrated distinct gene expression patterns in the two cell lines, including genes on chromosomes 12 and 19, which is consistent with the cytogenetic profile in this cell line. Finally, OSU-CLL was readily transplantable into NOG mice, producing uniform engraftment by three weeks with leukemic cells detectable in the peripheral blood spleen and bone marrow. These studies describe a new CLL cell line that extends currently available models to study gene function in this disease. |
| Databáze: | OpenAIRE |
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