Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects

Autor: M. Leonor Cancela, Tiziano Verri, Ivar Rønnestad, Alessandro Romano, Ann-Elise Olderbakk Jordal, Paulo J. Gavaia, Carla Viegas, Yuko Kamisaka, Tom Ole Nilsen
Přispěvatelé: I., Ronnestad, P. J., Gavaia, C. S. B., Viega, Verri, Tiziano, A., Romano, T. O., Nilsen, A. E. O., Jordal, Y., Kamisaka, M. L., Cancela
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Comparative sequence analysis
Physiology
Teleost
Molecular Sequence Data
Sequence alignment
Aquatic Science
Peptide Transporter 1
Complementary DNA
Tissue expression
Gadus
Animals
Humans
Tissue Distribution
Amino Acid Sequence
RNA
Messenger
Cloning
Molecular
Molecular Biology
Peptide sequence
Oligopeptide transporter PepT1
Ecology
Evolution
Behavior and Systematics
Phylogeny
chemistry.chemical_classification
biology
Base Sequence
Symporters
Peptide transporter 1
Sequence Analysis
DNA
Zebrafish Proteins
biology.organism_classification
Amino acid
comparative sequence analysi
Open reading frame
chemistry
Biochemistry
Amino Acid Substitution
Gadus morhua
Gene Expression Regulation
Insect Science
Peptide
biology.protein
Animal Science and Zoology
Atlantic cod
Digestive System
Oligopeptides
Sequence Alignment
Zdroj: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Popis: SUMMARY A novel full-length cDNA that encodes for the Atlantic cod (Gadus morhua L.) PepT1-type oligopeptide transporter has been cloned. This cDNA(named codPepT1) was 2838 bp long, with an open reading frame of 2190 bp encoding a putative protein of 729 amino acids. Comparison of the predicted Atlantic cod PepT1 protein with zebrafish, bird and mammalian orthologs allowed detection of many structural features that are highly conserved among all the vertebrate proteins analysed, including (1) a larger than expected area of hydrophobic amino acids in close proximity to the N terminus; (2) a single highly conserved cAMP/cGMP-dependent protein kinase phosphorylation motif; (3) a large N-glycosylation-rich region within the large extracellular loop; and (4) a conserved and previously undescribed stretch of 8–12 amino acid residues within the large extracellular loop. Expression analysis at the mRNA level indicated that Atlantic cod PepT1 is mainly expressed at intestinal level, but that it is also present in kidney and spleen. Analysis of its regional distribution along the intestinal tract of the fish revealed that PepT1 is ubiquitously expressed in all segments beyond the stomach,including the pyloric caeca, and through the whole midgut. Only in the last segment, which included the hindgut, was there a lower expression. Atlantic cod PepT1, the second teleost fish PepT1-type transporter documented to date,will contribute to the elucidation of the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, it can represent a useful tool for the study of gut functional regionalization, as well as a marker for the analysis of temporal and spatial expression during ontogeny.
Databáze: OpenAIRE
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