Expression of Bcl-x, Bcl-2, Bax, and Bak in endarterectomy and atherectomy specimens
| Autor: | Anurag Saxena, Dorothy Thomson, J. D. McMeekin |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Atherectomy
Coronary Pathology medicine.medical_specialty Arteriosclerosis bcl-X Protein Apoptosis Bcl-xL Pathology and Forensic Medicine Renal Artery Bcl-2-associated X protein Western blot Proto-Oncogene Proteins In Situ Nick-End Labeling medicine Humans bcl-2-Associated X Protein Endarterectomy Carotid TUNEL assay biology medicine.diagnostic_test Karyorrhexis Membrane Proteins Muscle Smooth Coronary Vessels Carotid Arteries bcl-2 Homologous Antagonist-Killer Protein Proto-Oncogene Proteins c-bcl-2 biology.protein Biomarkers Bcl-2 Homologous Antagonist-Killer Protein Pyknosis |
| Zdroj: | The Journal of Pathology. 196:335-342 |
| ISSN: | 1096-9896 0022-3417 |
| Popis: | The regulation of apoptosis in atherosclerosis is not completely defined. The aim of this study was to determine the expression of Bcl-2, Bcl-x, Bax, and Bak in relation to apoptosis in advanced atherosclerotic lesions. In atherectomy (15), endarterectomy (10), and control non-atherosclerotic segments of renal (2) and of coronary and carotid (5) arteries, the extent of apoptosis was determined using TdT dUTP nick end labelling (TUNEL) and nuclear morphology (karyorrhexis/pyknosis) and expression of apoptosis regulators by immunohistochemistry and western blot analysis on paraffin-embedded material. In all specimens, the atherosclerotic involvement was advanced: grade V (n=18) and grade VI (n=7). The apoptotic index was high (mean 30%) in advanced lesions compared with controls ( |
| Databáze: | OpenAIRE |
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