Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions
| Autor: | Viorica Railean-Plugaru, Justyna Walczak, Joanna Rudnicka, Bogusław Buszewski, Agnieszka Rogowska, Paweł Pomastowski |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
silver nanoparticles
Lactobacillus paracasei Health Toxicology and Mutagenesis Microorganism lcsh:Medicine 02 engineering and technology Saccharomyces cerevisiae Toxicology Article law.invention 03 medical and health sciences chemistry.chemical_compound Probiotic Biotransformation law Stress Physiological mycotoxins Spectroscopy Fourier Transform Infrared MALDI-TOF MS Food science Chromatography High Pressure Liquid 030304 developmental biology 0303 health sciences biology Lactococcus lactis lcsh:R Lacticaseibacillus paracasei probiotic microorganisms 021001 nanoscience & nanotechnology biology.organism_classification Yeast Lactic acid chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Zearalenone Adsorption 0210 nano-technology metabolism Bacteria |
| Zdroj: | Toxins Volume 11 Issue 8 Toxins, Vol 11, Iss 8, p 463 (2019) |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins11080463 |
| Popis: | The zearalenone binding and metabolization ability of probiotic microorganisms, such as lactic acid bacteria, Lactobacillus paracasei, Lactococcus lactis, and yeast Saccharomyces cerevisiae, isolated from food products, were examined. Moreover, the influence of cellular stress (induced by silver nanoparticles) and lyophilization on the effectiveness of tested microorganisms was also investigated. The concentration of zearalenone after a certain time of incubation with microorganisms was determined using high-performance liquid chromatography. The maximum sorption effectiveness for L. paracasei, L. lactis, and S. cerevisiae cultured in non-stress conditions was 53.3, 41.0, and 36.5%, respectively. At the same time for the same microorganisms cultured at cellular stress conditions, the maximum sorption effectiveness was improved to 55.3, 47.4, and 57.0%, respectively. Also, the effect of culture conditions on the morphology of the cells and its metabolism was examined using microscopic technique and matrix-assisted laser desorption ionization-time of flight mass spectrometry, respectively. |
| Databáze: | OpenAIRE |
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