Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean
| Autor: | Wensheng Xiang, Xiaoxin Zhuang, Xiaowei Guo, Yongjiang Wang, Zhiyin Yu, Chongxi Liu, Sheng-Xiong Huang, Zhiyan Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Microbiology (medical) Cellulosimicrobium 01 natural sciences Microbiology Streptomyces Article Actinobacteria 03 medical and health sciences diseased soybean root Virology Pathogen lcsh:QH301-705.5 EC50 biology actinobacterial community Sclerotinia sclerotiorum (Lib.) de Bary Kribbella Sclerotinia sclerotiorum antifungal activity biology.organism_classification 030104 developmental biology lcsh:Biology (General) new agroactive compounds Rhodococcus 010606 plant biology & botany |
| Zdroj: | Microorganisms, Vol 7, Iss 8, p 243 (2019) Microorganisms Volume 7 Issue 8 |
| ISSN: | 2076-2607 |
| Popis: | The present study was conducted to examine the influence of a pathogen Sclerotinia sclerotiorum (Lib.) de Bary on the actinobacterial community associated with the soybean roots. A total of 70 endophytic actinobacteria were isolated from the surface-sterilized roots of either healthy or diseased soybeans, and they were distributed under 14 genera. Some rare genera, including Rhodococcus, Kribbella, Glycomyces, Saccharothrix, Streptosporangium and Cellulosimicrobium, were endemic to the diseased samples, and the actinobacterial community was more diverse in the diseased samples compared with that in the heathy samples. Culture-independent analysis of root-associated actinobacterial community using the high-throughput sequencing approach also showed similar results. Four Streptomyces strains that were significantly abundant in the diseased samples exhibited strong antagonistic activity with the inhibition percentage of 54.1&ndash 87.6%. A bioactivity-guided approach was then employed to isolate and determine the chemical identity of antifungal constituents derived from the four strains. One new maremycin analogue, together with eight known compounds, were detected. All compounds showed significantly antifungal activity against S. sclerotiorum with the 50% inhibition (EC50) values of 49.14&ndash 0.21 mg/L. The higher actinobacterial diversity and more antifungal strains associated with roots of diseased plants indicate a possible role of the root-associated actinobacteria in natural defense against phytopathogens. Furthermore, these results also suggest that the root of diseased plant may be a potential reservoir of actinobacteria producing new agroactive compounds. |
| Databáze: | OpenAIRE |
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