The orphan receptor cDNA RDC4 encodes a 5-HT1D serotonin receptor
| Autor: | Frederic Libert, Carine Maenhaut, Herbert Ladinsky, Claude Massart, J. Van Sande, Gilbert Vassart, Jacques Emile Dumont, Ettore Giraldo, Christiane Dinsart, E. Monferini |
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| Rok vydání: | 1991 |
| Předmět: |
Tetrahydronaphthalenes
Biophysics Biology Transfection Binding Competitive Polymerase Chain Reaction Biochemistry Cell Line Beta-1 adrenergic receptor Estrogen-related receptor alpha GTP-Binding Proteins Muscarinic acetylcholine receptor M5 Cyclic AMP Enzyme-linked receptor Animals 5-HT5A receptor RNA Messenger Cloning Molecular Molecular Biology Orphan receptor 8-Hydroxy-2-(di-n-propylamino)tetralin Lysergic Acid DNA Cell Biology Molecular biology Recombinant Proteins Kinetics Receptors Serotonin Interleukin-21 receptor Adrenal Cortex Estrogen-related receptor gamma |
| Zdroj: | Biochemical and Biophysical Research Communications. 180:1460-1468 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(05)81360-9 |
| Popis: | The cDNA of RDC4, a putative receptor of the G protein-coupled receptor family, has been cloned by PCR methodology. The primary structure of this receptor showed homology with the serotonin 5-HT1A receptor. In this work, RDC4 mRNA has been injected in Y1 adrenal cells and Xenopus oocytes and RDC4 cDNA has been transfected transiently in cos-7 cells. In all these systems serotonin elicited a rise in cyclic AMP levels. Binding studies on membranes of the transfected cos-7 cells using [3H]-LSD showed a pattern of drug affinities consistent with the known properties of a 5-HT1D receptor. RDC4 therefore codes for a 5-HT1D receptor which in the studied systems is positively coupled to adenylate cyclase. |
| Databáze: | OpenAIRE |
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