MYB-like transcription factor NoPSR1 is crucial for membrane lipid remodeling under phosphate starvation in the oleaginous microalga Nannochloropsis oceanica
| Autor: | Hiroki Murakami, Natsue Kakutani, Koichi Hori, Yunato Kuroyanagi, Hiroyuki Ohta, Mie Shimojima, Masako Iwai |
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| Rok vydání: | 2020 |
| Předmět: |
Time Factors
Transcription Genetic Membrane lipids Biophysics Arabidopsis N-Acetylglucosaminyltransferases Biochemistry Phosphates 03 medical and health sciences chemistry.chemical_compound Membrane Lipids Protein Domains Structural Biology Genetics medicine Microalgae MYB Amino Acid Sequence Molecular Biology Transcription factor 030304 developmental biology Starvation 0303 health sciences medicine.diagnostic_test Chemistry 030302 biochemistry & molecular biology Algal Proteins Cell Biology Phosphate Lipid Metabolism Cell biology Membrane Gene Expression Regulation Mutation lipids (amino acids peptides and proteins) medicine.symptom Starvation response Lipid profile Transcription Factors |
| Zdroj: | FEBS lettersReferences. 594(20) |
| ISSN: | 1873-3468 |
| Popis: | Membrane lipid remodeling under phosphate (Pi) limitation, a process that replaces structural membrane phospholipids with nonphosphorus lipids, is a widely observed adaptive response in plants and algae. Here, we identified the transcription factor phosphorus starvation response 1 (NoPSR1) as an indispensable player for regulating membrane lipid conversion during Pi starvation in the microalga Nannochloropsis oceanica. Knocking out NoPSR1 scarcely perturbed membrane lipid composition under Pi-sufficient conditions but significantly impaired dynamic alteration in membrane lipids during Pi starvation. In contrast, the absence of NoPSR1 led to no obvious change in cell proliferation or storage lipid accumulation under either nutrient-sufficient or Pi-deficient conditions. Our results demonstrate a key factor controlling the membrane lipid profile during the Pi starvation response in N. oceanica. |
| Databáze: | OpenAIRE |
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