Isolation of Japanese encephalitis virus from Culex annulirostris in Australia
| Autor: | Scott A. Ritchie, Annette K. Broom, Michael Poidinger, Andrew F. van den Hurk, Debbie A. Phillips, John S. Mackenzie |
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| Rok vydání: | 1997 |
| Předmět: |
Sindbis virus
Culex annulirostris viruses Molecular Sequence Data Polymerase Chain Reaction Virus Disease Outbreaks Flaviviridae Aedes Virology Sequence Homology Nucleic Acid parasitic diseases medicine Animals Encephalitis Japanese Fluorescent Antibody Technique Indirect DNA Primers Encephalitis Virus Japanese biology Base Sequence Outbreak Japanese encephalitis biology.organism_classification medicine.disease Insect Vectors Flavivirus Culex Infectious Diseases Vector (epidemiology) DNA Viral RNA Viral Parasitology Queensland |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0002-9637 |
| Popis: | Mosquitoes were collected using CO and octenol-baited light traps during an outbreak of Japanese encephalitis (JE) on Badu Island in the Torres Strait, Australia in April 1995. A total of 13, 300 mosquitoes comprising 12 species were processed for virus isolation. Eight isolates of JE virus were obtained from Culex annulirostris, with a carriage rate of 2.97: 1, 000; this mosquito also yielded one Sindbis virus isolate. A reverse transcriptase-polymerase chain reaction was used to sequence the JE viruses, which were compared with published sequences. The eight isolates were 90% homologous with known JE strains but only 68% homologous with other flaviviruses. Among the isolates, 99% homology was obtained, suggesting a common point of origin. |
| Databáze: | OpenAIRE |
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