Potent Fungal Chitinase for the Bioconversion of Mycelial Waste
| Autor: | Han Hongyu, Yong Zhou, Tamo Fukamizo, Qing Yang, Xiaoguang Guo, Di Wang, Tian Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Autolysis (biology)
Bioconversion Gene Expression Bacillus subtilis Raw material Fungal Proteins chemistry.chemical_compound Chitin Food science Mycelium Waste Products biology Chemistry Hydrolysis Chitinases Aspergillus niger General Chemistry biology.organism_classification Biodegradation Environmental Chitinase Biocatalysis biology.protein General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 68:5384-5390 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.0c01342 |
| Popis: | Aspergillus niger mycelial waste is a good raw material for production of N-acetyl-d-glucosamine (GlcNAc). In this study, AnChiB, an A. niger chitinase which is upregulated during autolysis, was found to degrade A. niger mycelial waste with high efficiency. It could produce 1.45 mM (GlcNAc)2 in 8 h from raw mycelial waste, outperforming other chitinases, including bacterial SmChiA, human HsCht, and insect OfChtI and OfChi-h. The crystal structure of AnChiB was determined, and residues Trp106 and Trp118 were found to be important for the activity of AnChiB toward mycelial waste; mutation of either Trp106 or Trp118 into phenylalanine or alanine resulted in dramatically decreased activity. A recombinant strain of Bacillus subtilis was constructed to extracellularly produce AnChiB, and the culture supernatant was used to treat mycelial waste. This eco-friendly strategy could produce 3.7 mM of GlcNAc from 10 g of mycelial waste in 94 h with a yield of 71.3%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|