The human decapping scavenger enzyme DcpS modulates microRNA turnover
| Autor: | Michael A. Tones, Sandra Piquet, Eric Paquet, Gabriel D. Bossé, Oussama Meziane, Claude Robert, Martin J. Simard, Dominic Gagné |
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| Rok vydání: | 2015 |
| Předmět: |
Exonuclease
RNA Caps DCPS Blotting Western Molecular Sequence Data Active Transport Cell Nucleus Fluorescent Antibody Technique Biology medicine.disease_cause Article RNA interference microRNA Endoribonucleases medicine Gene silencing Humans Amino Acid Sequence Peptide sequence Oligonucleotide Array Sequence Analysis Cell Nucleus Mutation Multidisciplinary Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling HEK 293 cells Blotting Northern Molecular biology Cell biology MicroRNAs HEK293 Cells biology.protein RNA Interference |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The decapping scavenger enzyme DcpS is known for its role in hydrolyzing the cap structure following mRNA degradation. Recently, we discovered a new function in miRNA degradation activation for the ortholog of DcpS in C. elegans. Here we show that human DcpS conserves its role in miRNA turnover. In human cells, DcpS is a nucleocytoplasmic shuttling protein that activates miRNA degradation independently of its scavenger decapping activity in the cytoplasmic compartment. We also demonstrate that this new function for DcpS requires the contribution of the 5′-3′ exonuclease Xrn2. Our findings support a conserved role of DcpS as a modulator of miRNA turnover in animals. |
| Databáze: | OpenAIRE |
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