Prior Cryopreservation of Ex Vivo-Expanded Cord Blood Cells Is Not Detrimental to Engraftment as Measured in the NOD-SCID Mouse Model
| Autor: | Julie A. Wood, Robert E. Nordon, Marcus R. Vowels, Christopher G. Milross, Alison M. Rice, Jamie Case, Cathryn J. Collins |
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| Rok vydání: | 2001 |
| Předmět: |
Transplantation
Heterologous Immunology Cell Culture Techniques CD34 Antigens CD34 Stem cell factor Mice SCID Cryopreservation Andrology Mice Megakaryocyte Mice Inbred NOD medicine Animals Humans Progenitor cell Chemistry Graft Survival Hematopoietic Stem Cell Transplantation Hematology Fetal Blood medicine.anatomical_structure Blood Preservation Cord blood Leukocyte Common Antigens Female Bone marrow Cell Division Ex vivo |
| Zdroj: | Journal of Hematotherapy & Stem Cell Research. 10:157-165 |
| ISSN: | 1525-8165 |
| DOI: | 10.1089/152581601750098435 |
| Popis: | Cytokine-mediated expansion has been proposed and successfully used to facilitate engraftment post transplantation. This study examined whether cryopreservation following expansion has a detrimental effect on the ability of cells to engraft, using the NOD-SCID mouse model. Cord blood (CB) CD34(+) cells were incubated for 7 days with stem cell factor (SCF), flt-3 ligand (FL), and megakaryocyte growth and development factor (MGDF). Expanded CD34(+) cells were transplanted into NOD-SCID mice either fresh or following cryopreservation and thawing. After thawing, recovery of nucleated cells was 94%, of CD34 cells was 63%, and of day-14 progenitors was 17%. The loss of day-14 progenitor cells among the thawed expanded cells did not influence the kinetics of human engraftment in the mouse. Bone marrow (BM) of mice transplanted with thawed expanded CD34(+) cells (14 +/- 3.9%) showed significantly higher levels of human engraftment than mice transplanted with fresh expanded CD34(+) cells (1.5 +/- 0.5%, p = 0.0064). Thawed expanded CD34(+) cells had significantly higher SCID Engrafting Potential (SEP) than freshly expanded CD34(+) cells (p0.001). Results suggest that prior cryopreservation does not prevent expanded cells engrafting in NOD-SCID mice. |
| Databáze: | OpenAIRE |
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