IL-8 mRNA expression by in situ hybridisation in human pituitary adenomas
| Autor: | J. A. Royds, L. Baxter, W. R. Timperley, T. H. Jones, D. R. Cullen, M. E. R. Suliman |
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| Rok vydání: | 1999 |
| Předmět: |
Adenoma
Adult Male Pathology medicine.medical_specialty Pituitary gland Angiogenesis Endocrinology Diabetes and Metabolism Immunocytochemistry Biology Endocrinology Anterior pituitary Reference Values Internal medicine von Willebrand Factor medicine Null cell Humans Pituitary Neoplasms RNA Messenger Interleukin 8 In Situ Hybridization Aged Aged 80 and over Interleukin-8 General Medicine Middle Aged medicine.disease Immunohistochemistry medicine.anatomical_structure Pituitary Gland Female |
| Zdroj: | European Journal of Endocrinology. :155-158 |
| ISSN: | 1479-683X 0804-4643 |
| Popis: | Several cytokines have been shown to be expressed in normal and adenomatous pituitary tissue. Recently, interleukin-8 (IL-8) mRNA was identified by reverse transcription (RT)-PCR in each of a series of 17 pituitary tumours examined. We have investigated further the presence of IL-8 mRNA, using in situ hybridisation in two normal human anterior pituitary specimens and 25 human pituitary adenomas. IL-8 mRNA was not identified in either of the two normal pituitary specimens. Only three of the 25 adenomas were positive for IL-8 mRNA. In these three tumours, which included two null cell adenomas and one gonadotrophinoma, the majority of tumour cells (>90%) were positive for IL-8 mRNA. The remaining 22 adenomas were completely negative. There was no difference in tumour size or type between the IL-8 positive and the IL-8 negative tumours, and immunocytochemistry for von Willebrandt factor showed that the two groups were also similar in their degree of vascularisation. In conclusion, IL-8 mRNA was found in 12% of pituitary adenomas studied and was histologically identified within the tumour cells. In situ hybridisation is a more appropriate technique for assessing cytokine mRNA production by human pituitary tumours because RT-PCR may be too sensitive, identifying very small, possibly pathologically insignificant, quantities of mRNA that could be produced by supporting cells such as fibroblasts, endothelial cells or macrophages. |
| Databáze: | OpenAIRE |
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