A comparison of the hemolytic potential of Optison™ and Albunex® in whole human blood in vitro: acoustic pressure, ultrasound frequency, donor and passive cavitation detection considerations
| Autor: | Christopher Cox, Andrew A. Brayman, Teresa A Sherman, E. Carr Everbach, Ryan R Knapp, Morton W. Miller |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Acoustics and Ultrasonics Biophysics Contrast Media In Vitro Techniques Hemolysis Albumins medicine Humans Ultrasonics Radiology Nuclear Medicine and imaging Sound pressure Ultrasonography Fluorocarbons Radiological and Ultrasound Technology Human blood business.industry Ultrasound Echogenicity medicine.disease In vitro Blood Cavitation Erythrocyte Count Human erythrocytes business Biomedical engineering |
| Zdroj: | Ultrasound in Medicine & Biology. 27:709-721 |
| ISSN: | 0301-5629 |
| DOI: | 10.1016/s0301-5629(01)00356-8 |
| Popis: | This project tested the hypothesis that a "second-generation" ultrasound (US) contrast agent (Optison), offering extended echogenicity over that of its "first-generation" predecessor (Albunex), would have the greater potential for sonolysis of human erythrocytes in vitro. Whole human blood, obtained from apparently healthy donors, was anticoagulated and subsequently exposed in vitro to US in the presence of one of each or neither of the two US contrast agents. The US exposures were for 30 s and involved frequency (1.0, 2.2 and 3.4 MHz) and amplitude (approximately 2.8 to 0.38 MPa P(-)) regimens; pulse duration (200 micros) and interpulse interval (20 ms) were held constant. The data supported the hypothesis, with an overall ratio of approximately 2.5 for relative extent of background-corrected US-induced hemolysis of the Optison/Albunex regimens. Passive cavitation detection analyses corroborated the results obtained with hemolysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|