Carbohydrate-to-carbohydrate interactions between α2,3- linked sialic acids on α2 integrin subunits and asialo-GM1 underlie the bone metastatic behavior of LNCaP-derivative C4-2B prostate cancer cells
| Autor: | Marie-Ange Krzewinski-Recchi, Sophie Groux-Degroote, Séverine Van Slambrouck, Philippe Delannoy, Wim F.A. Steelant, Aurélie Cazet |
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| Přispěvatelé: | St. Thomas University, Miami, Saint Thomas University, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université Lille Nord de France (COMUE), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université de Lille-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Integrin alpha2 lcsh:Life lcsh:QR1-502 Biochemistry AsGM1 asialo-GM1 lcsh:Microbiology Collagen receptor chemistry.chemical_compound CT threshold cycle value 0302 clinical medicine SNA Sambucus nigra agglutinin CPI carbohydrate-to-protein interaction Neoplasm Metastasis bone metastasis 0303 health sciences ST sialyltransferase biology glycosphingolipid CCI carbohydrate-to-carbohydrate interaction IP immunoprecipitation ECM extracellular matrix carbohydrate–carbohydrate interaction Integrin alpha M FAK focal adhesion kinase 030220 oncology & carcinogenesis Integrin beta 6 lipids (amino acids peptides and proteins) GSL glycosphingolipid integrin Integrin MAA Maackia amurensis agglutinin collagen type I Biophysics Bone Neoplasms G(M1) Ganglioside 03 medical and health sciences sialylation Cell Line Tumor LNCaP TMA tissue microarray Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology 030304 developmental biology Original Paper QPCR quantitative PCR ALP alkaline phosphatase Prostatic Neoplasms Cell Biology Sialic acid carbohydrates (lipids) lcsh:QH501-531 chemistry NBT/BCIP Nitro Blue Tetrazolium/5-bromo-4-chloroindol-3-yl phosphate ST3GAL3 biology.protein Sialic Acids DIG digoxigenin |
| Zdroj: | Bioscience Reports Bioscience Reports, Portland Press, 2014, epub ahead of print. ⟨10.1042/BSR20140096⟩ Bioscience Reports, Vol 34, Iss 5, p e00138 (2014) Bioscience Reports, 2014, epub ahead of print. ⟨10.1042/BSR20140096⟩ |
| ISSN: | 0144-8463 1573-4935 |
| DOI: | 10.1042/BSR20140096⟩ |
| Popis: | Complex interplays among proteins, lipids and carbohydrates can alter the phenotype and are suggested to have a crucial role in tumour metastasis. Our previous studies indicated that a complex of the GSLs (glycosphingolipids), AsGM1 (asialo-GM1), which lacks α2,3-linked sialic acid, and α2β1 integrin receptors is responsible for the metastatic behaviour of C4-2B prostate cancer cells. Herein, we identified and addressed the functional significance of changes in sialylation during prostate cancer progression. We observed an increase in α2,3-linked sialic acid residues on α2 subunits of α2β1 integrin receptors, correlating with increased gene expression of α2,3-STs (sialyltransferases), particularly ST3GAL3. Cell surface α2,3-sialylation of α2 subunits was required for the integrin α2β1-dependent cell adhesion to collagen type I and the same α2,3-linked sialic acid residues on the integrin receptor were responsible for the interaction with the carbohydrate moiety of AsGM1, explaining the complex formation between AsGM1 and α2β1 integrin receptors. These results provide novel insights into the role of sialic acids in the organization and function of important membrane components in invasion and metastatic processes. |
| Databáze: | OpenAIRE |
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