Cations and the binding of polyadenylate to cellulose
| Autor: | G.S. King, Paul A. Kitos, Robert T. Hersh, T. Fuller |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Chemical Phenomena
Hofmeister series Polynucleotides Cesium Salt (chemistry) Fractionation Buffers Lithium Biochemistry Genetics and Molecular Biology (miscellaneous) Potassium Chloride chemistry.chemical_compound Ammonia Polymer chemistry Organic chemistry Polyadenylate Lignin Cellulose chemistry.chemical_classification Chromatography Binding Sites Adenine Nucleotides Chemistry Physical Elution Sodium Sodium Dodecyl Sulfate Cations Monovalent Molecular Weight chemistry Ionic strength Chromatography Gel Ultracentrifugation |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 353:362-374 |
| ISSN: | 0005-2787 |
| DOI: | 10.1016/0005-2787(74)90030-6 |
| Popis: | At neutral pH polyadenylate (poly(A)) binds to unmodified cellulose in high salt solution and is released in low salt solution. The salt effect is due principally to the cation, not to the ionic strength of the solution. Monovalent cations are not equally supportive of binding. The order of their effectiveness corresponds to a Hofmeister lyotropic series, increasing with decreasing radius of the hydrated cation. The cellulose · poly(A) interaction does not seem to be due to simple hydrophobic attraction but is consistent with association by π—π interaction between aromatic constituents of lignin of the cellulose and the pruine nuclei of the poly(A). Fractional elution of poly(A) from cellulose can be accomplished by gradually decreasing the salt content of the eluting buffer. The poly(A) oligomers which disengage from cellulose at rather high salt concentrations seem to be shorter than those which are released only at lower salt concentrations. This discrimination may serve as a basis for the fractionation of poly(A)-containing RNAs of which the poly(A) suffixes differ in length. |
| Databáze: | OpenAIRE |
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