Self-Transfer and Genetic Recombination Mediated by P, the Sex Factor of Vibrio cholerae
| Autor: | Charlotte Parker, W. R. Romig |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
DNA
Bacterial Genetics Microbial food.ingredient Kinetics Genetics and Molecular Biology medicine.disease_cause Microbiology Genetic recombination law.invention food law medicine Agar Mating Vibrio cholerae Molecular Biology Vibrio Recombination Genetic Bacteriological Techniques biology biology.organism_classification Culture Media Conjugation Genetic Recombinant DNA Recombination |
| Zdroj: | Journal of Bacteriology. 112:707-714 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.112.2.707-714.1972 |
| Popis: | Vibrio cholerae cells, infected with the sex factor P, produce discrete, plaque-like clearings when plated on lawns of P − cells. We investigated the nature of these clearings and conclude that they are probably sites of active mating. We developed a quantitative assay for P + cells and used it to study the kinetics of sex factor spread in broth cultures. Both established and newly infected donor populations were efficient sex factor donors, indicating that P is not self-repressed. We also investigated the kinetics of recombinant formation in broth matings. In 1-hr matings, we routinely found recombination frequencies of 10 −6 per donor cell. Kinetic studies of recombinant formation showed that the markers tested all appeared at early times. Thus P, the V. cholerae sex factor, seems to resemble F in its transfer properties. |
| Databáze: | OpenAIRE |
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