Association between human papilloma virus/Epstein-Barr virus coinfection and oral carcinogenesis

Autor: Xiaohua Rong, Rona S. Scott, Oleksandr Ekshyyan, Fleurette Abreo, Xin Gu, Mingxia Shi, Joseph T. Guidry, Eben L. Rosenthal, Ru Jiang, Sean Nathan, Tara Moore-Medlin, Lindsey M. Hutt-Fletcher, Cherie-Ann O. Nathan
Rok vydání: 2014
Předmět:
Epstein-Barr Virus Infections
Herpesvirus 4
Human
Cancer Research
Pathology
medicine.medical_specialty
Carcinogenesis
Palatine Tonsil
Tonsillar Neoplasms
Oropharynx
Alphapapillomavirus
Biology
medicine.disease_cause
Article
Virus
Palatine tonsil
Pathology and Forensic Medicine
Sleep Apnea Syndromes
Tongue
stomatognathic system
Cell Line
Tumor
hemic and lymphatic diseases
medicine
Humans
Neoplasm Invasiveness
Epstein–Barr virus infection
Cyclin-Dependent Kinase Inhibitor p16
Cell Proliferation
Palatal Neoplasms
Coinfection
Papillomavirus Infections
Cancer
medicine.disease
Epstein–Barr virus
Virology
Tongue Neoplasms
Oropharyngeal Neoplasms
Cell Transformation
Neoplastic
medicine.anatomical_structure
Otorhinolaryngology
Tonsil
Carcinoma
Squamous Cell
Periodontics
Receptors
Complement 3d
Palate
Soft
Oral Surgery
Zdroj: Journal of Oral Pathology & Medicine. 44:28-36
ISSN: 0904-2512
DOI: 10.1111/jop.12221
Popis: Background The recent epidemic of head and neck squamous cell carcinomas associated with human papilloma virus (HPV) has not addressed its association with lymphoid tissue in the oropharynx or the potential role of Epstein–Barr virus (EBV)/HPV coinfection. Methods The prevalence of HPV and EBV infection/coinfection and CD21 mRNA expression were determined in normal and cancerous tissues from the oropharynx using in situ hybridization (ISH), p16, and quantitative reverse transcriptase PCR (qRT-PCR). The effects of coinfection on tumorigenicity were evaluated using proliferation and invasion assays. Results Normal oropharynx, tonsil, non-cancer base of tongue (BOT), and BOT from sleep apnea patients demonstrated EBV positivity ranging from 7% to 36% depending on the site and methods of detection used (qRT-PCR or ISH). Among non-malignant BOT samples, HPV positivity was noted only in 20%. The percent of tonsil and BOT cancers positive for HPV (up to 63% and 80%, respectively) or coinfected with HPV/EBV (up to 25% and 70%, respectively) were both significantly associated with cancer status. Notably, HPV/EBV coinfection was observed only in malignant tissue originating in lymphoid-rich oropharynx sites (tonsil, BOT). CD21 mRNA (the major EBV attachment receptor) was detected in tonsil and BOT epithelium, but not in soft-palate epithelium. Coinfected cell lines showed a significant increase in invasiveness (P
Databáze: OpenAIRE
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