MagicplexTM Sepsis Real-Time test to improve bloodstream infection diagnostics in children
| Autor: | Marco, Denina, Carlo, Scolfaro, Colombo, Sara, Calitri, Carmelina, Garazzino, Silvia, Maria, Barbui Anna, Brossa, Silvia, Tovo, Pier Angelo, Manzoni, Paolo |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Time Factors 030106 microbiology One Hundred Fifty Bacteremia Sensitivity and Specificity Pediatrics Real-time polymerase chain reaction Sepsis Immunocompromised Host 03 medical and health sciences Interquartile range Internal medicine Diagnosis medicine Humans Blood culture Prospective Studies Child Prospective cohort study Intensive care medicine Children medicine.diagnostic_test business.industry Medical record Pediatrics Perinatology and Child Health Perinatology and Child Health medicine.disease 030104 developmental biology Child Preschool Etiology Female business |
| Zdroj: | European Journal of Pediatrics. 175:1107-1111 |
| ISSN: | 1432-1076 0340-6199 |
| DOI: | 10.1007/s00431-016-2745-3 |
| Popis: | Sepsis is the major cause of morbidity and mortality in children, especially in immunocompromised patients, and a rapid identification of causative pathogen is strongly related with a better outcome. This prospective study analyzes the role of a multiplex real-time polymerase chain reaction in sepsis' etiological diagnosis. Magicplex(TM) Sepsis Real-Time tests were performed in tertiary Regina Margherita Children's Hospital (Turin, Italy), and the medical records of children who underwent a Magicplex test were prospectively evaluated. Results of the Magicplex test were compared with those of blood culture collected at a close time point. One hundred fifty Magicplex tests were collected from 89 patients (54 males and 35 females, age interquartile range: 2.6-12.1 years). Etiological definition was achieved in 60 bloodstream infection cases (40 %). In 32 episodes, Magicplex test alone gave a positive result, and blood culture alone permitted the etiological diagnosis in 5 septic episodes. Magicplex test allowed a 143 % increase in the diagnostic value of blood cultures.These results suggest that molecular biology can be useful for rapid pathogen's identification also in children.• Sepsis represents a major cause of morbidity and mortality in children. • Sepsis outcome is strongly related to rapid microbiological identification and prompt initiation of an appropriate chemotherapy. What is New: • This manuscript is the first that describes the use of Magicplex (TM) Sepsis Real-Time test in children. • The results suggest that molecular biology can be useful for rapid pathogen's identification also in children. |
| Databáze: | OpenAIRE |
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