KLF13 Cooperates with c-Maf To Regulate IL-4 Expression in CD4+ T Cells
| Autor: | Alan M. Krensky, Carol Clayberger, Seok Joo Kwon, Juan Crespo-Barreto, Dong Seok Kim, Tianhong Wang, Wei Zhang |
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| Rok vydání: | 2014 |
| Předmět: |
Transcription
Genetic Immunology Kruppel-Like Transcription Factors KLF13 Cell Cycle Proteins Article CCL5 Mice Th2 Cells Animals Immunology and Allergy IL-2 receptor Promoter Regions Genetic STAT4 Interleukin 4 CXCL16 Interleukin 3 Mice Knockout Mice Inbred BALB C Chemistry GATA3 Molecular biology Repressor Proteins Gene Expression Regulation Proto-Oncogene Proteins c-maf Interleukin-4 |
| Zdroj: | J Immunol |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1302830 |
| Popis: | Kruppel-like factor (KLF) 13 is a transcription factor that positively regulates expression of the chemokine RANTES 3–5 d after activation of T cells. In this study, we document a key role for KLF13 in the expression of IL-4 in CD4+ T cells. Gene expression analysis in activated T cells from Klf13−/− mice showed that IL-4, along with other Th2 cytokine genes, was downregulated when compared with cells from wild-type mice. The decreased levels of IL-4 were not associated with changes in expression of the Th2-inducing transcription factors GATA3 or c-Maf. Additional analysis revealed that KLF13 directly binds to IL-4 promoter regions and synergizes with c-Maf to positively regulate IL-4 expression. These results indicate that KLF13 is a positive regulator for differentiation of Th2 cells, as part of the transcriptional machinery that regulates IL-4 production in Th2 cells. |
| Databáze: | OpenAIRE |
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