Quantitative Analysis of the Relative Transcript Levels of ABC Transporter Atr Genes in Aspergillus nidulans by Real-Time Reverse Transcription-PCR Assay
| Autor: | Camile P. Semighini, Maria Helena S. Goldman, Gustavo H. Goldman, Mozart Marins |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Antifungal Agents
Transcription Genetic Ecology biology Reverse Transcriptase Polymerase Chain Reaction Mutant Fungal genetics Wild type ATP-binding cassette transporter biology.organism_classification Applied Microbiology and Biotechnology Molecular biology Aspergillus nidulans Fungal Proteins Reverse transcription polymerase chain reaction Transcription (biology) Drug Resistance Multiple Fungal Mutation Methods ATP-Binding Cassette Transporters Gene Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 68:1351-1357 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | The development of assays for quantitative analysis of the relative transcript levels of ABC transporter genes by real-time reverse transcription-PCR (RT-PCR) might provide important information about multidrug resistance in filamentous fungi. Here, we evaluate the potential of real-time RT-PCR to quantify the relative transcript levels of ABC transporter Atr genes from Aspergillus nidulans . The AtrA to AtrD genes showed different and higher levels in the presence of structurally unrelated drugs, such as camptothecin, imazalil, itraconazole, hygromycin, and 4-nitroquinoline oxide. We also verified the relative transcript levels of the Atr genes in the A. nidulans imazalil-resistant mutants. These genes displayed a very complex pattern in different ima genetic backgrounds. The imaB mutant has higher basal transcript levels of AtrB and - D than those of the wild-type strain. The levels of these two genes are comparable when the imaB mutant is grown in the presence and absence of imazalil. The imaC , - D , and - H mutants have higher basal levels of AtrA than that of the wild type. The same behavior is observed for the relative transcript levels of AtrB in the imaG mutant background. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|