An Adhesive Secreted by Australian Frogs of the Genus Notaden
| Autor: | Michael J. Tyler, Lloyd D. Graham, Paul R. Vaughan, Veronica Glattauer, John Alan Maurice Ramshaw, Jerome Anthony Werkmeister, Yong Y. Peng |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
0106 biological sciences
0301 basic medicine Notaden Anatomy Biology Matrix (biology) Notaden bennettii biology.organism_classification 010603 evolutionary biology 01 natural sciences In vitro Cell biology 03 medical and health sciences 030104 developmental biology Tandem repeat In vivo Secretion Ex vivo |
| Zdroj: | Biological Adhesives ISBN: 9783540310488 Biological Adhesives ISBN: 9783319460819 |
| DOI: | 10.1007/978-3-540-31049-5_11 |
| Popis: | When provoked, the Australian fossorial frog Notaden bennettii secretes from its dorsal skin an exudate which rapidly forms a tacky elastic hydrogel. This protein-based material acts as a promiscuous pressure-sensitive adhesive which works even in wet conditions. The largest protein, Nb-1R, is rich in Gly, Pro/Hyp and Glx and appears to be the key structural component; it probably contains extensive segments of intrinsic disorder along with some well-folded domains. Indeed, the material properties of adhesive secretions from both amphibians (vertebrates) and onychophorans (invertebrates) may rely upon large proteins containing long and intrinsically unstructured regions composed of imperfect tandem repeats. Although the N. bennettii secretion contains sterols, carotenoids and other undesirable metabolites, in vitro, ex vivo and in vivo studies suggest that the structural matrix of the set glue is highly biocompatible. Its open porous structure is likely to encourage cell infiltration, and glue pellets implanted subcutaneously in mice were fully resorbed by the surrounding tissues within two months. Ex vivo studies in sheep showed that the frog glue bonded meniscal tears more strongly than traditional protein-based medical glues, while conventional tendon attachment repairs were approximately doubled in strength when augmented with the frog adhesive. Overall, the properties of the frog glue suggest that a recombinant mimic would have great potential for medical applications. |
| Databáze: | OpenAIRE |
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