m-AMSA as a probe for transport phenomena associated with anthracycline resistance
| Autor: | Candace Wheeler, David Kessel |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Drug
Naphthacenes media_common.quotation_subject Kinetics Drug Resistance Antineoplastic Agents Biochemistry Cell Line chemistry.chemical_compound Mice medicine Animals Amsacrine media_common Pharmacology HEPES Antibiotics Antineoplastic Aminoacridines Leukemia P388 Biological Transport medicine.disease Leukemia Aminacrine chemistry Cell culture Acridine Biophysics Intracellular medicine.drug |
| Zdroj: | Biochemical pharmacology. 33(7) |
| ISSN: | 0006-2952 |
| Popis: | Kinetics of transport of the acridine derivative 4'-(9-acridinylamino)-methanesulfon-m-anisidide (m-AMSA) were examined in P388 murine leukemia cells and in P388/ADR, a subline selected for adriamycin resistance and cross-resistant to a variety of drugs including m-AMSA. Compared with the drug-responsive parent cell line, P388/ADR cells showed impaired accumulation of m-AMSA and an enhanced rate of drug exodus. Competition studies demonstrated structural specificity of the outward transport process. There was a low degree of intracellular m-AMSA binding, and steady-state drug levels were reached in less than 1 min. These results suggest that m-AMSA will be a useful probe for studying transport systems associated with anthracycline resistance. |
| Databáze: | OpenAIRE |
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