Chloroquine Allows to Distinguish between Hepatocyte Lysosomes and Sinusoidal Cell Lysosomes
| Autor: | S. Wattiaux-De Coninck, F. Gentinne, Robert Wattiaux, F. Dubois, Michel Jadot |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Biophysics Cell Fractionation Biochemistry Cathepsin C Chloroquine Lysosome Centrifugation Density Gradient medicine Animals Rats Wistar Bovine serum albumin Molecular Biology Differential centrifugation biology Acid phosphatase Cell Biology Rats medicine.anatomical_structure Liver Hepatocyte biology.protein Cell fractionation Lysosomes Subcellular Fractions medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 190:808-813 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.1121 |
| Popis: | We have examined the effect of chloroquine on rat liver lysosomal enzyme distributions after isopycnic centrifugation in a sucrose gradient. Chloroquine injection causes the large majority of cathepsin C, acid phosphatase and N acetyl glucosaminidase to migrate towards lower density regions; on the other hand only about 50% of arylsulfatase and acid deoxyribonuclease are subjected to such a density shift. To specifically mark hepatocyte lysosomes and sinusoidal cell lysosomes, rats were injected with galactosylated bovine serum albumin (A) or mannosylated bovine serum albumin (B) labelled with 125I tyramine cellobiose; A is selectively endocytosed by hepatocytes, B by sinusoidal cells. The radioactivity distribution is affected by chloroquine in the same way as cathepsin C, after injection of A though it is not influenced by chloroquine after the injection of B. These results show that chloroquine does not modify the density of liver sinusoidal cell lysosomes when it decreases the density of hepatocyte lysosomes. Such a difference could result from the fact that sinusoidal cell lysosomes do not accumulate chloroquine to the same extent as hepatocyte lysosomes. Chloroquine treatment could be useful to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes. |
| Databáze: | OpenAIRE |
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