Cryptosporidium parvum in Korea: Prevalence in Individuals Residing in Three Major River Valleys and Genetic Characteristics of the Isolates
| Autor: | Hyeng-Il Cheun, Isao Kimata, Byung-Chul Lee, Shin-Hyeong Cho, Tong-Soo Kim, Byoung-Kuk Na, Jung-Won Ju, Jung-Yeon Kim, Jae-Ran Yu, Yi-Young Lim |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Rural Population Adolescent Cryptosporidium infection Cryptosporidiosis Polymerase Chain Reaction 18S ribosomal RNA Microbiology Apicomplexa Feces Young Adult Sex Factors Zoonoses parasitic diseases Prevalence RNA Ribosomal 18S medicine Animals Humans Parasite hosting Child Parasite Egg Count Aged Aged 80 and over Cryptosporidium parvum Chi-Square Distribution Korea General Veterinary biology Age Factors Infant Cryptosporidium DNA Protozoan Middle Aged Ribosomal RNA biology.organism_classification medicine.disease Child Preschool Female Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Veterinary Medical Science. 72:167-172 |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | Cryptosporidiosis is a diarrheal illness caused by apicomplexa parasite Cryptosporidium spp. In this study, to examine the overall infection status of Cryptosporidium spp. in individuals residing in southern parts of Korea, eight counties around Yeongsan, Seomjin and Nakdong River valleys was surveyed. The investigation was carried out from April to October 2005. A total of 9,498 stool samples were collected from individuals. Stool samples were analyzed for modified acid-fast stains, and DNA fragment extracted from positive samples was identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for 18S rRNA polymorphic region. Oocysts of Cryptosporidium spp. were detected in 239 specimens (2.5%) by a modified acid-fast stain. Infection rate was not significantly different between male (2.2%) and female (2.8%) individuals examined (P>0.05). In the infection rate by age, totally 1-9 (4.8%) and 80< (3.7%) age group were shown to the highest, and there was shown to significant differences (P |
| Databáze: | OpenAIRE |
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