Quantitation of ultraviolet radiation-induced cyclobutyl pyrimidine dimers in DNA by video and photographic densitometry
| Autor: | Bryan D. Thompson, Steven E. Freeman |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Pyrimidine
Ultraviolet Rays Video Recording Biophysics Pyrimidine dimer Biochemistry chemistry.chemical_compound Image Processing Computer-Assisted Photography Fluorometry Molecular Biology Electrophoresis Agar Gel Gel electrophoresis Dose-Response Relationship Radiation DNA Cell Biology Molecular biology Fluorescence chemistry Pyrimidine Dimers Agarose gel electrophoresis Densitometry Ethidium bromide Analog-Digital Conversion DNA Damage Nuclear chemistry |
| Zdroj: | Analytical Biochemistry. 186:222-228 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(90)90070-p |
| Popis: | We have compared video and photographic methods for calculating the number of ultraviolet radiation (uv)-induced pyrimidine dimers in DNA from the bacteriophage T7 exposed to uv (0 to 800 J/m2) from an FS40 sunlamp. DNA was incubated with a pyrimidine dimer-specific Micrococcus luteus uv endonuclease, subjected to alkaline agarose gel electrophoresis, neutralized, and stained with ethidium bromide, and the DNA fluorescence was recorded either with a video camera or on photographic film. The slopes of the dose-response curves for the number of uv-endonuclease-sensitive sites per 10(3) bases (pyrimidine dimers) was 1.2 (+/- 0.1) X 10(-4) uv-endonuclease-sensitive sites per J/m2 for the video analysis and 1.3 (+/- 0.04) X 10(-4) uv-endonuclease-sensitive sites per J/m2 for the photographic analysis. Results for pyrimidine dimer determination by either method were statistically comparable. |
| Databáze: | OpenAIRE |
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