Effect of human umbilical cord blood cells on Ang-II-induced hypertrophy in mice
| Autor: | Sravan K. Vanamala, Jasti S. Rao, Christopher S. Gondi, Sreelatha Gopinath |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Cellular differentiation medicine.medical_treatment Biophysics Apoptosis Cardiomegaly Biology Biochemistry Umbilical cord Article Muscle hypertrophy Mice Fibrosis Internal medicine medicine Animals Humans Myocytes Cardiac Molecular Biology Angiotensin II Stem Cells Histocompatibility Antigens Class I Cell Differentiation Cell Biology Stem-cell therapy medicine.disease Fetal Blood Coculture Techniques Rats medicine.anatomical_structure Endocrinology Heart failure Immunology Stem cell Biomarkers Stem Cell Transplantation |
| Zdroj: | Biochemical and biophysical research communications. 386(2) |
| ISSN: | 1090-2104 |
| Popis: | We have assessed the capacity of human umbilical cord blood (hUCB)-derived stem cells to differentiate into cardiomyocytes and repair angiotensin II induced insult in culture and in mouse hearts when injected. hUCB were able to differentiate into cardiomyocyte-like cells, when induced with 5-azacytidine or co-cultured with rat neonatal cardiomyocytes (NRCM). When co-cultured, hUCB reversed the pathological effects induced by angiotensin II (Ang-II) in NRCM and in mice injected after Ang-II infusion. As assessed by increased heart weight to body mass ratio and Ang-II-induced fibrosis, cardiac hypertrophy was also reduced after hUCB were injected. hUCB also reversed the pathological heart failure markers induced by Ang-II in mice. Further, we observed a shift from pathological hypertrophy towards physiological hypertrophy by hUCB in Ang-II-challenged mice. Our findings support hUCB as a feasible model for experimentation in stem cell therapy and emphasize the relevance of the hUCB in reversing heart failure conditions. |
| Databáze: | OpenAIRE |
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