Superior anticancer activity is demonstrated by total extract of Curcuma longa L. as opposed to individual curcuminoids separated by centrifugal partition chromatography
| Autor: | Shakti Upadhyay, Ewelina Gumbarewicz, Kazimierz Głowniak, Ewa Nowosadzka, Andrzej Stepulak, Aneta Grabarska, Gregoire Audo, Lidia Czernicka, Agata Jarząb, Wirginia Kukula-Koch, Jarogniew J. Łuszczki |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Curcumin Coumaric Acids Chemical Fractionation Mass Spectrometry Ferulic acid 03 medical and health sciences chemistry.chemical_compound Curcuma 0302 clinical medicine Diarylheptanoids Cell Line Tumor Neoplasms Bisdemethoxycurcumin medicine Humans Chromatography High Pressure Liquid Pharmacology Cisplatin Chromatography biology Plant Extracts biology.organism_classification Antineoplastic Agents Phytogenic 030104 developmental biology chemistry A549 Cells Cell culture 030220 oncology & carcinogenesis Cancer cell Solvents Zingiberaceae HT29 Cells Phytotherapy medicine.drug |
| Zdroj: | Phytotherapy Research. 32:933-942 |
| ISSN: | 0951-418X |
| DOI: | 10.1002/ptr.6035 |
| Popis: | Three curcuminoids: bisdemethoxycurcumin, demethoxycurcumin, and curcumin from turmeric were successfully separated by a high capacity solvent system composed of heptane: chloroform: methanol: water mixture (5: 6: 3: 2 v/v/v/v) tailored for centrifugal partition chromatographs at K-values of 0.504, 1.057, 1.644, respectively. These three ferulic acid derivatives obtained at a purity rate exceeding 95% were analysed by an HPLC-MS spectrometer. Turmeric extract inhibited the proliferation/viability of A549 human lung cancer, HT29 colon cancer, and T98G glioblastoma cell lines in (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay (MTT). Single curcuminoids significantly decreased the viability/proliferation of lung cancer cells in a dose-dependent manner. However, total extract displayed the superior anticancer activity in the investigated cell lines. Crude extract in combination with cisplatin augmented the decrease in the viability of cancer cells compared with single compound treatment in A549 lung cancer cells. Total extract of Curcuma longa could be regarded as being more effective against lung cancer cells in vitro than its separated compounds. |
| Databáze: | OpenAIRE |
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