Biofilm propensity of Staphylococcus aureus skin isolates is associated with increased atopic dermatitis severity and barrier dysfunction in the MPAACH pediatric cohort
| Autor: | Mariana L. Stevens, Lisa J. Martin, Daniel Spagna, Tammy Gonzalez, John Kroner, Hua He, Brittany Grashel, Asel Baatyrbek Kyzy, Jocelyn M. Biagini Myers, Andrew B. Herr, Gurjit K. Khurana Hershey, Rosario Alarcon, Elaine Sidler |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
0301 basic medicine Staphylococcus aureus Immunology Filaggrin Proteins medicine.disease_cause Article Dermatitis Atopic Microbiology 03 medical and health sciences 0302 clinical medicine Staphylococcus epidermidis medicine Humans Immunology and Allergy Child Barrier function Skin Transepidermal water loss business.industry Biofilm Atopic dermatitis Staphylococcal Infections biochemical phenomena metabolism and nutrition Antimicrobial medicine.disease 030104 developmental biology medicine.anatomical_structure 030228 respiratory system Biofilms Keratinocyte business Filaggrin |
| Zdroj: | Allergy |
| ISSN: | 1398-9995 0105-4538 |
| Popis: | Background Atopic dermatitis (AD) patients are often colonized with Staphylococcus aureus, and staphylococcal biofilms have been reported on adult AD skin lesions. The commensal S epidermidis can antagonize S aureus, although its role in AD is unclear. We sought to characterize S aureus and S epidermidis colonization and biofilm propensity and determine their associations with AD severity, barrier function, and epidermal gene expression in the first US early-life cohort of children with AD, the Mechanisms of Progression of Atopic Dermatitis to Asthma in Children (MPAACH). Methods The biofilm propensity of staphylococcal isolates was assessed by crystal violet assays. Gene expression of filaggrin and antimicrobial alarmins S100A8 and S100A9 was measured in keratinocyte RNA extracted from skin tape strips. Staphylococcal biofilms sampled from MPAACH skin were visualized using scanning electron microscopy. Results Sixty-two percent of staphylococcal isolates (sampled from 400 subjects) formed moderate/strong biofilms. Sixty-eight percent of subjects co-colonized with both staphylococcal species exhibited strains that formed cooperative mixed-species biofilms. Scanning electron microscopy verified the presence of staphylococcal biofilms on the skin of MPAACH children. Staphylococcus aureus strains showing higher relative biofilm propensity compared with S epidermidis were associated with increased AD severity (P = .03) and increased lesional and nonlesional transepidermal water loss (P = .01, P = .03). Conclusions Our data suggest a pathogenic role for S aureus biofilms in AD. We found that strain-level variation in staphylococcal isolates governs the interactions between S epidermidis and S aureus and that the balance between these two species, and their biofilm propensity, has important implications for AD. |
| Databáze: | OpenAIRE |
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