High-sensitivity simultaneous liquid chromatographyâtandem mass spectrometry assay of ethinyl estradiol and levonorgestrel in human plasma
| Autor: | Priti Trivedi, Swati Guttikar, Abhishek Gandhi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Electrospray
Formic acid Pharmaceutical Science Pharmacy Levonorgestrel Mass spectrometry Analytical Chemistry chemistry.chemical_compound LC–MS/MS Liquid chromatography–mass spectrometry Drug Discovery Electrochemistry medicine Derivatization Spectroscopy Ethinyl estradiol Medicine(all) Chromatography Biochemistry Genetics and Molecular Biology(all) lcsh:RM1-950 Solvent lcsh:Therapeutics. Pharmacology chemistry Linear range Human plasma Original Article medicine.drug |
| Zdroj: | Journal of Pharmaceutical Analysis, Vol 5, Iss 5, Pp 316-326 (2015) Journal of Pharmaceutical Analysis |
| ISSN: | 2095-1779 |
| Popis: | A sensitive and simultaneous liquid chromatographyâtandem mass spectrometry method was developed and validated for quantification of ethinyl estradiol and levonorgestrel. The analytes were extracted with methyl-tert-butyl ether: n-hexane (50:50, v/v) solvent mixture, followed by dansyl derivatization. The chromatographic separation was performed on a Kinetex C18 (50 mmÃ4.6 mm, 2.6 µm) column with a mobile phase of 0.1% (v/v) formic acid in water and acetonitrile in gradient composition. The mass transitions were monitored in electrospray positive ionization mode. The assay exhibited a linear range of 0.100â20.0 ng/mL for levonorgestrel and 4.00â500 pg/mL for ethinyl estradiol in human plasma. A run time of 9.0 min for each sample made it possible to analyze a throughput of more than 100 samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic and bioequivalence studies. Keywords: Ethinyl estradiol, Levonorgestrel, LCâMS/MS, Human plasma, Derivatization |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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