Uncoupling protein 2 mRNA expression and respiratory parameters in Kupffer cells isolated from euthyroid and hyperthyroid rat livers
| Autor: | Ilaria Demori, F Goglia, Emilia Fugassa, AT Franzi, Adriana Voci, Antonia Lanni |
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| Přispěvatelé: | Voci, A, Demori, I, Franzi, At, Fugassa, E, Goglia, F, Lanni, Antonia |
| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty endocrine system diseases Kupffer Cells Cellular respiration Endocrinology Diabetes and Metabolism Gene Expression Mitochondrion Biology Hyperthyroidism Ion Channels Mitochondrial Proteins Oxygen Consumption Endocrinology Internal medicine Respiration medicine Animals Uncoupling protein Uncoupling Protein 2 Euthyroid RNA Messenger Rats Wistar Respiratory system Body Weight Kupffer cell Membrane Transport Proteins Proteins General Medicine Rats medicine.anatomical_structure Liver Triiodothyronine Respiration rate |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1479-683X 0804-4643 |
| DOI: | 10.1530/eje.0.1450317 |
| Popis: | OBJECTIVE: The levels of uncoupling protein 2 (UCP2) mRNA and determinants of respiration (ATP synthesis, proton leak and non-mitochondrial respiration) were evaluated in Kupffer cells isolated from the livers of normal euthyroid, acute hyperthyroid and chronic hyperthyroid rats. METHODS: After liver perfusion, Kupffer cells were purified by density-gradient centrifugation followed by counterflow centrifugal elutriation. UCP2 mRNA levels were measured by Northern blot and respiratory parameters by polarographic method. RESULTS: In cells isolated from hyperthyroid (tri-iodothyronine (T(3))-treated) rats, the effect of T(3) treatment on the UCP2 mRNA level varied: it was more than doubled (P |
| Databáze: | OpenAIRE |
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