Comparative proteomic analysis of differentially expressed proteins in primary retinoblastoma tumors
| Autor: | Perinkulam Ravi Deepa, Vikas Khetan, Yogendra Sharma, Curam Sreenivasacharlu Sundaram, Lingam Gopal, Tarun Sharma, Subramanian Krishnakumar, Jyotirmay Biswas, Kandalam Mallikarjuna |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Proteomics Clinical Biochemistry Biology medicine.disease_cause Recoverin Gene expression Biomarkers Tumor medicine Humans Electrophoresis Gel Two-Dimensional Aged Aged 80 and over Regulation of gene expression Reverse Transcriptase Polymerase Chain Reaction Retinoblastoma Middle Aged medicine.disease Immunohistochemistry Molecular biology Gene Expression Regulation Neoplastic Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Female Apolipoprotein A1 Carcinogenesis |
| Zdroj: | PROTEOMICS - Clinical Applications. 4:449-463 |
| ISSN: | 1862-8346 |
| Popis: | Purpose: To understand the disease mechanism and to identify the potential tumor markers that would help in therapeutics, comparative proteomic analysis of 29 retinoblastoma (RB) tumors was performed using 14 non-neoplastic retinas (age ranged from 45 to 89 years) as control tissues. Experimental design: 2-DE and MALDI-TOF-TOF MS/MS were used to identify differentially expressed proteins. Results: Twenty-seven distinct differentially expressed proteins were identified, including 16 upregulated 11 downregulated proteins. Significantly, higher mRNA levels of apolipoprotein A1 (po0.001), transferrin (TF; po0.001), CRABP2 (po0.001), a-crystallin A (CRYAA; po0.001) were observed in RBs when compared with normal retinas and hence are consistent with the proteomic data. Immunohistochemistry was also performed for selected proteins on paraffin RB blocks to confirm protein expression. RB with invasion showed significantly higher expression by 2-DE-MS/MS analysis of CRABP2 (po0.001), peroxiredoxin 6 (p 50.025), apolipoprotein A1 (po0.001), recoverin (po0.001). Conclusions and clinical relevance: Thus, this study provides a dynamic protein profile of RB tumors, which could provide clues to study the mechanisms of RB oncogenesis and possibly be developed as potential biomarkers for prognosis and therapy. |
| Databáze: | OpenAIRE |
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