Exploration of the role of the virulence factor ElrA during Enterococcus faecalis cell infection

Autor: Stéphane Gaubert, Jean-Marie Herry, Romain Briandet, Julien Deschamps, Pascale Serror, Natalia Nunez, Yu Wei, Thomas Baranek, Mustapha Si-Tahar, Aurélie Derré-Bobillot, Cristel Archambaud
Přispěvatelé: Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Département de Virologie - Department of Virology, Institut Pasteur [Paris] (IP), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), N.N. was supported by a fellowship from the French Ministère de l’Enseignement Supérieur et de la Recherche. P.S. thanks the French-Brazilian USP COFECUB project Uc Me 147/13 for traveling support., Institut Pasteur [Paris], AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS), Réseau International des Instituts Pasteur (RIIP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours, Wei, Yu
Rok vydání: 2018
Předmět:
0301 basic medicine
[SDV]Life Sciences [q-bio]
Cell
lcsh:Medicine
MESH: Virulence
Virulence factor
Mice
Enterococcus faecalis
MESH: RAW 264.7 Cells
Macrophage
MESH: Animals
lcsh:Science
MESH: Bacterial Proteins
Multidisciplinary
Virulence
biology
Hep G2 Cells
medicine.anatomical_structure
MESH: Caco-2 Cells
MESH: Enterococcus faecalis
MESH: Cell Line
Tumor
Virulence Factors
030106 microbiology
MESH: Hep G2 Cells
Article
Cell Line
Microbiology
03 medical and health sciences
Bacterial Proteins
Leucine
Cell Line
Tumor
medicine
Extracellular
Animals
Humans
MESH: Staphylococcal Protein A
Staphylococcal Protein A
MESH: Mice
Gram-Positive Bacterial Infections
MESH: Virulence Factors
MESH: Humans
Macrophages
lcsh:R
MESH: Macrophages
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Cell Line
FHL2
RAW 264.7 Cells
MESH: Leucine
MESH: HeLa Cells
biology.protein
lcsh:Q
MESH: Gram-Positive Bacterial Infections
Caco-2 Cells
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Protein A
HeLa Cells
Zdroj: Scientific Reports
Scientific Reports, Nature Publishing Group, 2018, 8 (1), ⟨10.1038/s41598-018-20206-6⟩
Scientific Reports, 2018, 8 (1), pp.1749. ⟨10.1038/s41598-018-20206-6⟩
Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.1749. ⟨10.1038/s41598-018-20206-6⟩
Scientific Reports 1749 (8), . (2018)
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
ISSN: 2045-2322
DOI: 10.1038/s41598-018-20206-6
Popis: Enterococcus faecalis, an organism generally not pathogenic for healthy humans, has the potential to cause disease in susceptible hosts. While it seems to be equipped to interact with and circumvent host immune defense, most of the molecular and cellular mechanisms underlying the enterococcal infectious process remain elusive. Here, we investigated the role of the Enterococcal Leucine Rich protein A (ElrA), an internalin-like protein of E. faecalis also known as a virulence factor. ElrA was previously shown to prevent adhesion to macrophages. We show that ElrA does not inhibit the basic phagocytic process, but is able to prevent sensing and migration of macrophages toward E. faecalis. Presence or absence of FHL2, a eukaryotic partner of ElrA, does not affect the ElrA-dependent mechanism preventing macrophage migration. However, we highlight a partial contribution of FHL2 in ElrA-mediated virulence in vivo. Our results indicate that ElrA plays at least a dual role of which anti-phagocytic activity may contribute to dissemination of extracellular E. faecalis during infection.
Databáze: OpenAIRE
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