A disposable biosensor employing a glucose-sensitive biochemomechanical gel
| Autor: | Ayumi Kumagai, Hiroaki Suzuki |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Hot Temperature
Immobilized enzyme Polymers Biomedical Engineering Biophysics Biosensing Techniques Silicone rubber Glucose Oxidase chemistry.chemical_compound Electrochemistry Glucose oxidase Shrinkage Acrylic acid chemistry.chemical_classification Acrylamides Chromatography biology Chemistry General Medicine Polymer Hydrogen-Ion Concentration Glucose Acrylates biology.protein Poly(N-isopropylacrylamide) Gels Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 18:1289-1297 |
| ISSN: | 0956-5663 |
| Popis: | A novel approach to construct a disposable biosensor is proposed. By immobilizing glucose oxidase in a pH-sensitive copolymerized poly(N-isopropylacrylamide)/acrylic acid gel, a glucose-sensitive gel can be obtained. When the gel makes contact with a sample solution containing glucose, the pH of the gel decreases as a result of the enzymatic reaction, inducing the collapse of the gel. The response time to the shrinkage depended upon the activity of the enzyme and the concentration of glucose, although the final ratio of shrinkage settled to approximately the same value irrespective of the concentration of glucose. The transient stage can be used for sensing. The volume change of the gel was transduced into the movement of a colored solution in a flow channel with the help of a silicone rubber diaphragm. The mechanism was used to construct a disposable glucose sensor. A clear dependence of the column length of the colored solution on the concentration of glucose was observed. By measuring the change at a predetermined time, the glucose concentration was obtained. This sensor can be considered the ultimate style in disposable sensors, as detection does not require any electrical measurement or spectroscopic procedures. |
| Databáze: | OpenAIRE |
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