Investigation of squalene-doxorubicin distribution and interactions within single cancer cell using Raman microspectroscopy

Autor: Hamid Morjani, Abdelilah Beljebbar, Almar Al Assaad, Patrick Couvreur, Andrei Maksimenko, Maité Callewaert, Hassan Rammal, Didier Desmaële, Laurence Van Gulick, Barbara Stella, Simona Mura, Franco Dosio
Přispěvatelé: Institut Galien Paris-Saclay (IGPS), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Biospectroscopie Translationnelle - EA 7506 (BIOSPECT), Université de Reims Champagne-Ardenne (URCA)
Rok vydání: 2021
Předmět:
Lung Neoplasms
Pharmaceutical Science
Medicine (miscellaneous)
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
02 engineering and technology
Spectrum Analysis
Raman
Mice
squalenoylated doxorubicin
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
polycyclic compounds
General Materials Science
Cancer cells
Nanoparticles
Raman microspectroscopy
Squalenoylated doxorubicin
0303 health sciences
Chemistry
021001 nanoscience & nanotechnology
3. Good health
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biophysics
medicine.anatomical_structure
symbols
Molecular Medicine
Nanomedicine
Female
Single-Cell Analysis
0210 nano-technology
[SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Intracellular
medicine.drug
Squalene
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Biomedical Engineering
Breast Neoplasms
[SDV.CAN]Life Sciences [q-bio]/Cancer
Bioengineering
macromolecular substances
03 medical and health sciences
symbols.namesake
Cell Line
Tumor
medicine
Animals
Humans
Doxorubicin
030304 developmental biology
organic chemicals
technology
industry
and agriculture
Subcellular localization
carbohydrates (lipids)
Cytoplasm
Cancer cell
[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic
cancer cells
Biophysics
nanoparticles
Raman spectroscopy
Nucleus
Zdroj: Nanomedicine: Nanotechnology, Biology and Medicine
Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2021, 35, pp.102404. ⟨10.1016/j.nano.2021.102404⟩
ISSN: 1549-9634
Popis: International audience; Intracellular distribution of doxorubicin (DOX) and its squalenoylated (SQ-DOX) nanoparticles (NPs) form in murine lung carcinoma M109 and human breast carcinoma MDA-MB-231 cells was investigated by Raman microspectroscopy. Pharmacological data showed that DOX induced higher cytotoxic effect than SQ-DOX NPs. Raman data were obtained using single-point measurements and imaging on the whole cell areas. These data showed that after DOX treatment at 1 μM, the spectral features of DOX were not detected in the M109 cell cytoplasm and nucleus. However, the intracellular distribution of SQ-DOX NPs was higher than DOX in the same conditions. In addition, SQ-DOX NPs were localized into both cell cytoplasm and nucleus. After 5 μM treatment, Raman bands of DOX at 1211 and 1241 cm-1 were detected in the nucleus. Moreover, the intensity ratio of these bands decreased, indicating DOX intercalation into DNA. However, after treatment with SQ-DOX NPs, the intensity of these Raman bands increased. Interestingly, with SQ-DOX NPs, the intensity of 1210/1241 cm-1 ratio was higher suggesting a lower fraction of intercalated DOX in DNA and higher amount of non-hydrolyzed SQ-DOX. Raman imaging data confirm this subcellular localization of these drugs in both M109 and MDA-MB-231 cells. These finding brings new insights to the cellular characterization of anticancer drugs at the molecular level, particularly in the field of nanomedicine.
Databáze: OpenAIRE
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