Rebuilding core abscisic acid signaling pathways of Arabidopsis in yeast
| Autor: | Stefanie V. Tischer, Julia Mergner, Isabel Doch, David Chiasson, Erwin Grill, Philippe Schmitt-Kopplin, Jörg Kudla, Stefanie Mucha, Kai H. Edel, Daniel Hemmler, Michael Papacek, Bernhard Kuster, Moritz Ruschhaupt |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Osmotic shock
Arabidopsis Protein Serine-Threonine Kinases Protein Engineering General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Transcription (biology) Gene Expression Regulation Plant Osmotic Pressure Yeasts News & Views Phosphorylation Molecular Biology Abscisic acid Transcription factor 030304 developmental biology 0303 health sciences General Immunology and Microbiology biology Abiotic stress Arabidopsis Proteins Abscisic Acid Abf Abiotic Stress Clade A Pp2c Rcar Pyl Pyr1 Snrk2 organic chemicals General Neuroscience fungi food and beverages biology.organism_classification Cell biology ddc Biosynthetic Pathways Metabolic pathway chemistry Signal transduction 030217 neurology & neurosurgery |
| Zdroj: | EMBO J EMBO J. 38:e101859 (2019) |
| ISSN: | 1460-2075 |
| Popis: | The phytohormone abscisic acid (ABA) regulates plant responses to abiotic stress, such as drought and high osmotic conditions. The multitude of functionally redundant components involved in ABA signaling poses a major challenge for elucidating individual contributions to the response selectivity and sensitivity of the pathway. Here, we reconstructed single ABA signaling pathways in yeast for combinatorial analysis of ABA receptors and coreceptors, downstream-acting SnRK2 protein kinases, and transcription factors. The analysis shows that some ABA receptors stimulate the pathway even in the absence of ABA and that SnRK2s are major determinants of ABA responsiveness by differing in the ligand-dependent control. Five SnRK2s, including SnRK2.4 known to be active under osmotic stress in plants, activated ABA-responsive transcription factors and were regulated by ABA receptor complexes in yeast. In the plant tissue, SnRK2.4 and ABA receptors competed for coreceptor interaction in an ABA-dependent manner consistent with a tight integration of SnRK2.4 into the ABA signaling pathway. The study establishes the suitability of the yeast system for the dissection of core signaling cascades and opens up future avenues of research on ligand-receptor regulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text