A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors
| Autor: | Aviv Regev, Donald M. Bryant, Duygu Payzin-Dogru, Nicholas D. Leigh, M. B. Couger, Tzu-Hsing Kuo, Sevara Bryant, Tia DiTommaso, Brian J. Haas, William W. Ye, Francis G. Davis, Kimberly Johnson, Clifford J. Tabin, Timothy L. Tickle, Steven Coyne, Leonid Peshkin, Stephanie L. Tsai, Tae J. Lee, Robert Freeman, Joel Bateman, Anna R. Guzikowski, Jessica L. Whited |
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| Přispěvatelé: | Broad Institute of MIT and Harvard, Regev, Aviv, Haas, Brian |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Trinity Morpholino kazald1 RNA Splicing axolotl Computational biology RNA Small Interfering/metabolism CIRBP Genome General Biochemistry Genetics and Molecular Biology Article Transcriptome Insulin-Like Growth Factor Binding Proteins/antagonists & inhibitors 03 medical and health sciences 0302 clinical medicine Axolotl Animals Regeneration RNA/chemistry expression analysis RNA Small Interfering blastema lcsh:QH301-705.5 In Situ Hybridization limb Genetics Trinotate cirbp biology Sequence Analysis RNA Regeneration (biology) RNA-Binding Proteins Extremities biology.organism_classification RNA-Binding Proteins/genetics Ambystoma mexicanum Insulin-Like Growth Factor Binding Proteins Extremities/physiology 030104 developmental biology lcsh:Biology (General) RNA RNA Interference Blastema Developmental biology 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Elsevier Cell Reports; 18(3), pp 762-776 (2017) Cell Reports, Vol 18, Iss 3, Pp 762-776 (2017) |
| ISSN: | 2211-1247 |
| Popis: | Mammals have extremely limited regenerative capabilities; however, axolotls are profoundly regenerative and can replace entire limbs. The mechanisms underlying limb regeneration remain poorly understood, partly because the enormous and incompletely sequenced genomes of axolotls have hindered the study of genes facilitating regeneration. We assembled and annotated a de novo transcriptome using RNA-sequencing profiles for a broad spectrum of tissues that is estimated to have near-complete sequence information for 88% of axolotl genes. We devised expression analyses that identified the axolotl orthologs of cirbp and kazald1 as highly expressed and enriched in blastemas. Using morpholino anti-sense oligonucleotides, we find evidence that cirbp plays a cytoprotective role during limb regeneration whereas manipulation of kazald1 expression disrupts regeneration. Our transcriptome and annotation resources greatly complement previous transcriptomic studies and will be a valuable resource for future research in regenerative biology. United States. National Institutes of Health (1U24CA180922-01) |
| Databáze: | OpenAIRE |
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