ZnO nanowire-based fluorometric enzymatic assays for lactate and cholesterol
| Autor: | M. Briones, María Encarnación Lorenzo, Félix Pariente, Andrés Redondo-Cubero, Carlos Busó-Rogero, Sergio Catalán-Gómez, Tania García-Mendiola |
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| Rok vydání: | 2020 |
| Předmět: |
Detection limit
chemistry.chemical_classification Photoluminescence Cholesterol oxidase Nanochemistry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Analytical Chemistry Enzyme catalysis chemistry.chemical_compound Cholesterol Enzyme chemistry Fluorometry Lactic Acid Zinc Oxide 0210 nano-technology Hydrogen peroxide Nuclear chemistry |
| Zdroj: | Repositorio Institucional del Instituto Madrileño de Estudios Avanzados en Nanociencia instname |
| ISSN: | 1436-5073 0026-3672 |
| Popis: | A rapid fluorometric method is described for the determination of lactate and cholesterol by using ZnO nanowires (ZnO NWs). The assay is based on the detection of the hydrogen peroxide generated during the enzymatic reactions of the oxidation of lactate or cholesterol. Taking advantage of the electrostatic interactions between the enzymes and the ZnO NWs, two bioconjugates were prepared by mixing the nanomaterial and the enzymes, viz. lactate oxidase (LOx) or cholesterol oxidase (ChOx). The enzymatically generated hydrogen peroxide quenches the fluorescence of the ZnO NWs, which have emission peaks at 384 nm and at 520 nm under 330 nm photoexcitation. H2O2 quenches the 520 nm band more strongly. Response is linear up to 1.9 μM lactate concentration, and up to 1.1 μM cholesterol concentration. Relative standard deviation was found to be 5%. The detection limits for lactate and cholesterol are 0.54 and 0.24 μM, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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