A novel sterol-binding protein reveals heterogeneous cholesterol distribution in neurite outgrowth and in late endosomes/lysosomes

Autor:	Akiko Yamaji-Hasegawa, Motohide Murate, Takehiko Inaba, Naoshi Dohmae, Masayuki Sato, Fumihiro Fujimori, Yasushi Sako, Peter Greimel, Toshihide Kobayashi
Rok vydání:	2021
Předmět:	Pharmacology Cellular and Molecular Neuroscience Sterols Cholesterol Neuronal Outgrowth Molecular Medicine Cell Biology Endosomes Carrier Proteins Lysosomes Molecular Biology
Zdroj:	Cellular and molecular life sciences : CMLS. 79(6)
ISSN:	1420-9071
Popis:	We identified a mushroom-derived protein, maistero-2 that specifically binds 3-hydroxy sterol including cholesterol (Chol). Maistero-2 bound lipid mixture in Chol-dependent manner with a binding threshold of around 30%. Changing lipid composition did not significantly affect the threshold concentration. EGFP-maistero-2 labeled cell surface and intracellular organelle Chol with higher sensitivity than that of well-established Chol probe, D4 fragment of perfringolysin O. EGFP-maistero-2 revealed increase of cell surface Chol during neurite outgrowth and heterogeneous Chol distribution between CD63-positive and LAMP1-positive late endosomes/lysosomes. The absence of strictly conserved Thr-Leu pair present in Chol-dependent cytolysins suggests a distinct Chol-binding mechanism for maistero-2.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::444e6d4ba1037f17d02936448aac80f4 https://pubmed.ncbi.nlm.nih.gov/35644822 Zobrazit plný text záznamu Full text from SpringerLink