Skeletal Muscle Signaling Following Whole-Body and Localized Heat Exposure in Humans
Autor: | Florian A Britto, Mohammed Ihsan, Louise Deldicque, John Molphy, Anissa Cherif, Sebastien Racinais |
---|---|
Přispěvatelé: | UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
muscle atrophy medicine.medical_specialty mitochondrial biogenesis Anabolism Physiology heat shock protein 030204 cardiovascular system & hematology lcsh:Physiology Muscle hypertrophy 03 medical and health sciences 0302 clinical medicine Atrophy Physiology (medical) Heat shock protein Internal medicine medicine PI3K/AKT/mTOR pathway Original Research lcsh:QP1-981 Chemistry heat treatment Skeletal muscle medicine.disease Muscle atrophy 030104 developmental biology Endocrinology medicine.anatomical_structure Mitochondrial biogenesis muscle mass medicine.symptom hypertrophy |
Zdroj: | Frontiers in Physiology Frontiers in Physiology, Vol 11 (2020) Frontiers in physiology, Vol. 11, no.-, p. 839 (2020) |
ISSN: | 1664-042X |
Popis: | This study identified the changes in hypertrophy/atrophy and mitochondrial-related signaling in human skeletal muscle following whole-body (WB) and localized single leg (SL) heat treatment. Nine active male participants were administered either 60 min of passive WB (44–50°C, 50% humidity) or SL (water-perfused suit at 49.5 ± 1.4°C) heat treatment at least 1 week apart in a counterbalanced order. The untreated leg during SL was considered as control (CON). Core, skin, and quadriceps muscle temperature were monitored throughout the experimental trials. Muscle microbiopsy samples were obtained prior to (PRE), and 30 min and 3 h post (POST) following heat treatment. Muscle temperature increased with time (p < 0.0001) in both WB and SL, with no differences between conditions (38.8 ± 0.5°C vs. 38.1 ± 0.6°C, p = 0.065). Core temperature increased only following WB, and was significantly higher compared with SL (39.1 ± 0.3°C vs. 37.1 ± 0.1, p < 0.0001). Compared with PRE, WB up-regulated the phosphorylation status of the majority of the Akt/mTOR pathway (Akt, mTOR, S6K1, rpS6, and p-eIF4E; p ≤ 0.050), with the exception of 4EBP1 (p = 0.139). WB also increased the mRNA of HSPs 72, 90, and 25 (all p < 0.021), and increased or tended to increase the phosphorylation of FOXO1 (p = 0.066) and FOXO3a (p = 0.038). In addition, most (NRF1, NRF2, COX2, and COX4-I2; all p ≤ 0.050), but not all (CS, Cyt c, and COX4-I1; p > 0.441) mRNA content indicative of mitochondrial biogenesis were increased following WB, with no changes evident in these parameters in SL or CON (all p > 0.090). These results indicate that 1 h of WB heat treatment enhanced anabolic (Akt/mTOR), mitochondrial, and cyto-protective signaling (HSP), with a concomitant possible inhibition of FOXO transcription factors. |
Databáze: | OpenAIRE |
Externí odkaz: |