Antibody response using six different serological assays in a completely PCR-tested community after a coronavirus disease 2019 outbreak—the CoNAN study
| Autor: | Michael Bauer, Gabi Hanf, Steffi Kolanos, Michael Baier, H Proquittè, Mathias W. Pletz, André Scherag, Juliane Scholz, Michael Kiehntopf, Christine Pinkwart, Local Cooperation Partners, Stefan Glöckner, Nico Andreas, Stefan Hagel, Sebastian Weis, Oliwia Makarewicz, Christina Bahrs, Aurelia Kimmig, Juliane Ankert, Raphaela Marquardt, Renate Koch, Franziska Röstel, Annika Licht, Jennifer Kosenkow, Petra Dickmann, Matthias Ullrich, Clara Schnizer, Stefanie Deinhardt-Emmer, Wibke Wetzker, Joel Guerra, Dagmar Rimek, Sebastian Kuhn, Lara Thieme, Cornelius Eibner, Jasmin Müller, Thomas Hotz, Thomas Kamradt, Cora Richert, Bettina Meinung, Steffen Mai, Anita Hartung, Petra Enders, Kay Stötzer, Julia Köhler, Bettina Löffler, Daniel Weiss, Hans Cipowicz |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine viruses Antibodies Viral Serology Cohort Studies 0302 clinical medicine Seroepidemiologic Studies Germany Medicine 030212 general & internal medicine skin and connective tissue diseases Child Asymptomatic Infections Aged 80 and over education.field_of_study biology General Medicine Middle Aged Infectious Diseases COVID-19 Nucleic Acid Testing Child Preschool Antibody response Quarantine RNA Viral Female Original Article Antibody medicine.symptom Cohort study Adult Microbiology (medical) medicine.medical_specialty Adolescent 030106 microbiology Population Asymptomatic Virus COVID-19 Serological Testing Young Adult 03 medical and health sciences Internal medicine Humans Seroprevalence education Aged SARS-CoV-2 business.industry Immunity COVID-19 Infant Outbreak Immunoglobulin G biology.protein business |
| Zdroj: | Clinical Microbiology and Infection |
| ISSN: | 1198-743X |
| DOI: | 10.1016/j.cmi.2020.11.009 |
| Popis: | Objectives Due to a substantial proportion of asymptomatic and mild courses, many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections remain unreported. Therefore, assessment of seroprevalence may detect the real burden of disease. We aimed to determine and characterize the rate of SARS-CoV-2 infections and the resulting seroprevalence in a defined population. The primary objective of the study was to assess SARS-CoV-2 antibody seroprevalence using six different IgG-detecting immunoassays. Secondary objectives of the study were: (a) to determine potential risk factors for symptomatic versus asymptomatic coronavirus disease 2019 courses, and (b) to investigate the rate of virus RNA-persistence. Methods CoNAN is a population-based cohort study performed in the community Neustadt am Rennsteig, Germany, which was quarantined from 22 March to 5 April after six SARS-CoV-2 cases were detected in the village's population. The SARS-CoV-2 outbreak comprised 51 cases and 3 deaths. The CoNAN study was performed from 13 May to 22 May 2020, 6 weeks after a SARS-CoV-2 outbreak. Results We enrolled a total of 626 participants (71% of the community population) for PCR and antibody testing in the study. All actual SARS-CoV-2 PCR tests were negative. Fifty-two out of 620 (8.4%) participants had antibodies against SARS-CoV-2 in at least two different assays. There were 38 participants with previously PCR-confirmed SARS-CoV-2 infection. Of those, only 19 (50%) displayed anti-SARS-CoV-2 antibodies. We also show that antibody-positive participants with symptoms compatible with a respiratory tract infection had significantly higher antibody levels then asymptomatic participants (EU-assay: median 2.9 versus 7.2 IgG-index, p 0.002; DS-assay: median 45.2 versus 143 AU/mL, p 0.002). Persisting viral replication was not detected. Conclusions Our data question the relevance and reliability of IgG antibody testing to detect past SARS-CoV-2 infections 6 weeks after an outbreak. We conclude that assessing immunity for SARS-CoV-2 infection should not rely on antibody tests alone. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|