Spread of Highly Pathogenic Avian Influenza (HPAI) H5N5 Viruses in Europe in 2016–2017 Appears Related to the Timing of Reassortment Events

Autor: Alex Bossers, Rene Heutink, Saskia A. Bergervoet, Cynthia K. Y. Ho, Nancy Beerens
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Time Factors
animal diseases
viruses
Reassortment
lcsh:QR1-502
genetic analysis
medicine.disease_cause
Genetic analysis
lcsh:Microbiology
Disease Outbreaks
Genotype
Influenza A Virus
H5N8 Subtype
Cells
Cultured
Phylogeny
Netherlands
Bacteriologie
virus diseases
Bacteriology
Host Pathogen Interaction & Diagnostics
Virology & Molecular Biology
Europe
Infectious Diseases
Ducks
Influenza A virus
Reassortant Viruses
animal structures
Highly pathogenic
030106 microbiology
Animals
Wild
Biology
complex mixtures
Virus
Article
03 medical and health sciences
Spatio-Temporal Analysis
Virology
medicine
Animals
highly pathogenic avian influenza
Epizootic
Host Pathogen Interaction & Diagnostics
Bacteriology
medicine.disease
Pathogenicity
Influenza A virus subtype H5N1
Host Pathogen Interactie & Diagnostiek
Virologie & Moleculaire Biologie
030104 developmental biology
Influenza in Birds
Bacteriologie
Host Pathogen Interactie & Diagnostiek
reassortment
avian influenza
Chickens
Zdroj: Viruses, Vol 11, Iss 6, p 501 (2019)
Viruses
Volume 11
Issue 6
Viruses 11 (2019) 6
Viruses, 11(6)
ISSN: 1999-4915
Popis: During the epizootic of highly pathogenic avian influenza (HPAI) H5N8 virus in Europe in 2016&ndash
2017, HPAI viruses of subtype H5N5 were also isolated. However, the detection of H5N5 viruses was limited compared to H5N8. In this study, we show that the genetic constellation of a newly isolated H5N5 virus is different from two genotypes previously identified in the Netherlands. The introduction and spread of the three H5N5 genotypes in Europe was studied using spatiotemporal and genetic analysis. This demonstrated that the genotypes were isolated in distinguishable phases of the epizootic, and suggested multiple introductions of H5N5 viruses into Europe followed by local spread. We estimated the timing of the reassortment events, which suggested that the genotypes emerged after the start of autumn migration. This may have prevented large-scale spread of the H5N5 viruses on wild bird breeding sites before introduction into Europe. Experiments in primary chicken and duck cells revealed only minor differences in cytopathogenicity and replication kinetics between H5N5 genotypes and H5N8. These results suggest that the limited spread of HPAI H5N5 viruses is related to the timing of the reassortment events rather than changes in virus pathogenicity or replication kinetics.
Databáze: OpenAIRE
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