Matrix metalloproteinase-19 in capillary endothelial cells: expression in acutely, but not in chronically, inflamed synovium
| Autor: | Ulrich Krawinkel, Radislav Sedlacek, Cornelia Kolb, Simon Mauch |
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| Rok vydání: | 1999 |
| Předmět: |
Pathology
medicine.medical_specialty Endothelium Angiogenesis Knee Injuries Biology Arthritis Rheumatoid Vasculogenesis Bursitis Matrix Metalloproteinases Secreted medicine Humans Tissue Inhibitor of Metalloproteinase-2 Synovitis Tissue Inhibitor of Metalloproteinase-1 Arthritis Synovial Membrane Metalloendopeptidases Cell Biology Capillaries Vascular endothelial growth factor B Endothelial stem cell Vascular endothelial growth factor A medicine.anatomical_structure Vascular endothelial growth factor C Immunology Acute Disease Chronic Disease Human umbilical vein endothelial cell Endothelium Vascular |
| Zdroj: | Experimental cell research. 250(1) |
| ISSN: | 0014-4827 |
| Popis: | Matrix metalloproteinase-19 (MMP-19), originally isolated as an autoantigen from the synovium of a patient suffering from rheumatoid arthritis (RA), is expressed in smooth muscle cells of the tunica media of large blood vessels of an RA patient, but not in the endothelial cell layer. By contrast, in acutely inflamed tissue, synovial capillaries strongly express MMP-19 in the cytoplasm, as shown by immunofluorescence of cryostat sections. In MMP-19-producing capillaries the beta3 integrin chain was found at the endothelial cell surface, as was the vascular endothelial cell growth factor receptor-2 (KDR). The specific tissue inhibitor of metalloproteinases TIMP-1 was absent or faintly stained in MMP-19-expressing capillaries, whereas TIMP-1, but not TIMP-2, was strongly expressed in large vessels and in MMP-19-negative capillaries of RA synovia. In the spontaneously transformed human umbilical vein endothelial cell line ECV304 neither MMP-19 transcripts nor protein could be detected. By contrast, primary cultures of human endothelial cells of either dermal or adipose tissue origin produced MMP-19 mRNA and protein. The results strongly suggest the regulated induction of matrix metalloproteinase-19 in capillary endothelial cells during acute inflammation and hint at a role of MMP-19 in angiogenesis. |
| Databáze: | OpenAIRE |
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