Vitamin D decreases Porphyromonas gingivalis internalized into macrophages by promoting autophagy
| Autor: | Xue Yang, Hongyan Wang, Qian Li, Xiaolin Tang, Chunliang Ma, Li Niu, Chunling Pan, Fengxue Geng, Shuangshuang Chen |
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| Rok vydání: | 2020 |
| Předmět: |
Autophagosome
Calcitriol Endogeny 03 medical and health sciences 0302 clinical medicine Western blot Lysosome polycyclic compounds medicine Autophagy Vitamin D General Dentistry Porphyromonas gingivalis medicine.diagnostic_test biology Chemistry Macrophages Autophagosomes Colocalization 030206 dentistry biology.organism_classification Cell biology medicine.anatomical_structure Otorhinolaryngology 030220 oncology & carcinogenesis lipids (amino acids peptides and proteins) medicine.drug |
| Zdroj: | Oral diseasesREFERENCES. 27(7) |
| ISSN: | 1601-0825 |
| Popis: | OBJECTIVES This paper aims to study the effect of the active form of vitamin D (calcitriol) on the internalized Porphyromonas gingivalis in macrophages and to assess the role of autophagy during this process. MATERIALS AND METHODS Quantitative RT-PCR and bacteria culture were used to quantify live P. gingivalis internalized into U937-derived macrophages. Western blot assays were performed to detect the effect of P. gingivalis and calcitriol on autophagy in macrophages. Transmission electron microscope was used to observe the effect of calcitriol on the status of internalized P. gingivalis. Colocalization of P. gingivalis with the autophagosome and lysosome markers was observed by confocal laser scanning microscopy. RESULTS Calcitriol caused a dose-dependent decrease in live P. gingivalis numbers and promoted both the endogenous and P. gingivalis-induced autophagy in macrophages. Calcitriol significantly promoted the destruction of P. gingivalis and the colocalization of P. gingivalis with autophagosome and lysosome markers. Conversely, with 3-MA, live P. gingivalis numbers in macrophages increased significantly and inhibition effect of calcitriol on the number of live P. gingivalis was attenuated. CONCLUSION In U937-derived macrophages, calcitriol may promote colocalization of P. gingivalis with autophagosomes and lysosomes, namely autophagy process, to degrade live P. gingivalis. |
| Databáze: | OpenAIRE |
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