Gold Nanoparticles Capped with Polyethyleneimine for Enhanced siRNA Delivery
| Autor: | Jin-Zhi Du, Jun Wang, Wen-Jing Song, Pei-Zhuo Zhang, Tianmeng Sun |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Small interfering RNA
Materials science SiRNA binding Blotting Western Green Fluorescent Proteins Metal Nanoparticles Apoptosis Cell Cycle Proteins macromolecular substances Protein Serine-Threonine Kinases Biomaterials RNA interference Cell Line Tumor Proto-Oncogene Proteins Humans Polyethyleneimine General Materials Science Gene Silencing RNA Small Interfering Gene knockdown Gene Transfer Techniques technology industry and agriculture RNA General Chemistry Cell sorting Molecular biology Colloidal gold Cell culture Biophysics Gold Biotechnology |
| Zdroj: | Small. 6:239-246 |
| ISSN: | 1613-6829 1613-6810 |
| DOI: | 10.1002/smll.200901513 |
| Popis: | An efficient and safe delivery system for small interfering RNA (siRNA) is required for clinical application of RNA interfering therapeutics. Polyethyleneimine (PEI)-capped gold nanoparticles (AuNPs) are successfully manufactured using PEI as the reductant and stabilizer, which bind siRNA at an appropriate weight ratio by electrostatic interaction and result in well-dispersed nanoparticles with uniform structure and narrow size distribution. With siRNA binding, PEI-capped AuNPs induce more significant and enhanced reduction in targeted green fluorescent protein expression in MDA-MB-435s cells, though more internalized PEI/siRNA complexes in cells are evidenced by confocal laser scanning microscopy observation and fluorescence-activated cell sorting analyses. PEI-capped AuNPs/siRNA targeting endogenous cell-cycle kinase, an oncogene polo-like kinase 1 (PLK1), display significant gene expression knockdown and induce enhanced cell apoptosis, whereas it is not obvious when the cells are treated with PLK1 siRNA using PEI as the carrier. Without exhibiting cellular toxicity, PEI-capped AuNPs appear to be suitable as a potential carrier for intracellular siRNA delivery. |
| Databáze: | OpenAIRE |
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